@article{32844553c9fc4b46bb9b66557771ed08,
title = "3H-flunitrazepam binding to bovine retina and the effect of GABA thereon",
abstract = "Bovine retinae were examined for their ability to bind 3H-flunitrazepam. Whole retinal homogenates revealed specific, high affinity, and saturable 3H-flunitrazepam binding (Kd, 1.1 nM; Bmax, 19.5 fmoles/mg tissue). In washed membrane preparations, 10 μM GABA caused a dramatic decrease in the Kd (42 percent). The rank order of potency for the inhibition of 3H-flunitrazepam binding by different benzodiazepines was: clonazepam > clobazam ≫ Ro 5-4864. These results are supportive for the presence of benzodiazepine receptors in the bovine retina and indicate a likeness with the previously described benzodiazepine receptors of mammalian brain.",
author = "Regan, {John W.} and Roeske, {William R.} and Yamamura, {Henry I.}",
note = "Funding Information: Acknowledgements Henry I. Yamamura is a recipient of a United States Public Health Service Research Scientist Development Award, Type-II (MH-00095) from the National Tnstitutes of Mental Health. Portions of this research were supported by United States Public Health Service Grants MH-27257, MH-30626, and Program Project Grant HL-20984. We would like to thank Hoffman-LaRoche, Inc. for the clonazepam and the Ro S-4864. We would also like to thank Ms. Carol Corelis for secretarial assistance in typing of this manuscript. Portions of this work were presented at the Symposium on the Mechanism of Action of the Bensodiazepines. (August 27, 1979, A.S.P.E.T. meeting).",
year = "1980",
month = apr,
doi = "10.1016/0028-3908(80)90195-1",
language = "English (US)",
volume = "19",
pages = "413--414",
journal = "Neuropharmacology",
issn = "0028-3908",
publisher = "Elsevier Ltd",
number = "4",
}