Success and efficiency of cell seeding in Avian Tendon Xenografts – A promising alternative for tendon and ligament reconstruction

Simon Thönnes, Peter Shelton, Daniel N. Bracey, Mark Van Dyke, Patrick Whitlock, Thomas L. Smith, Arash Moghaddam, Christopher Tuohy

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Decellularized tendon xenografts offer a promising alternative for reconstruction by using ubiquitously available material. This study compares static and centrifugal seeding of avian tendon scaffolds with NIH 3T3 fibroblasts. Incorporation of viable cells was achievable with both techniques, represented by DNA content. Proliferation rate and viability assay showed neither damage by centrifugal force nor superiority of the technique. Cell proliferation after 10 days of culture demonstrated that the scaffold did not hinder 3-D culturing. Confocal laser microscopy revealed structural details as formation of focal adhesions, to provide deeper insight into the process of cell attachment and growth in xenografts.

Original languageEnglish (US)
Pages (from-to)155-161
Number of pages7
JournalJournal of Orthopaedics
Volume18
DOIs
StatePublished - Mar 1 2020
Externally publishedYes

Keywords

  • Cell adhesion
  • Cell seeding efficiency
  • Confocal laser microscopy
  • Tendon scaffold
  • Tissue engineering

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

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