Substrate specificity and membrane topologies of the iron-containing ω3 and ω6 desaturases from Mortierella alpina

Mingxuan Wang, Haiqin Chen, Aisikaer Ailati, Wei Chen, Floyd H. Chilton, W. Todd Lowther, Yong Q. Chen

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Polyunsaturated fatty acids (PUFAs) are essential lipids for cell function, normal growth, and development, serving as key structural components of biological membranes and modulating critical signal transduction events. Omega-3 (n-3) long chain PUFAs (LC-PUFAs) such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) have been shown to protect against inflammatory diseases and enhance brain development and function. This had led to a marked increase in demand for fish and fish oils in human diets, supplements, and aquaculture and created a need for new, sustainable n-3 LC-PUFA sources. We have studied for the first time homogenous preparations of the membrane-type ω6 and ω3 fatty acid desaturases from the fungus Mortierella alpina, as a model system to produce PUFAs. These desaturases possess a di-iron metal center and are selective for 18:1 n-9 and 18:2 n-6 acyl-CoA substrates, respectively. Sequence alignments and membrane topology predictions support that these enzymes have unique cap regions that may include the rearrangement and repositioning of the active site, especially when compared to the mammalian stearoyl–coenzyme A desaturase-1 (SCD1) and the related sphingolipid α-hydroxylase (Scs7p) that act upon different substrates.

Original languageEnglish (US)
Pages (from-to)211-223
Number of pages13
JournalApplied Microbiology and Biotechnology
Volume102
Issue number1
DOIs
StatePublished - Jan 1 2018
Externally publishedYes

Keywords

  • Enzyme kinetics
  • Enzyme purification
  • Fatty acid desaturase
  • Lipid
  • Polyunsaturated fatty acid (PUFA)
  • Regioselectivity

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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