@article{f20f6357ea294b79a93c2920a1c96ff1,
title = "Subdiffraction-resolution fluorescence microscopy reveals a domain of the centrosome critical for pericentriolar material organization",
abstract = "As the main microtubule-organizing centre in animal cells, the centrosome has a fundamental role in cell function. Surrounding the centrioles, the pericentriolar material (PCM) provides a dynamic platform for nucleating microtubules. Although the importance of the PCM is established, its amorphous electron-dense nature has made it refractory to structural investigation. By using SIM and STORM subdiffraction-resolution microscopies to visualize proteins critical for centrosome maturation, we demonstrate that the PCM is organized into two main structural domains: a layer juxtaposed to the centriole wall, and proteins extending farther away from the centriole organized in a matrix. Analysis of Pericentrin-like protein (PLP) reveals that its carboxy terminus is positioned at the centriole wall, it radiates outwards into the matrix and is organized in clusters having quasi-nine-fold symmetry. By RNA-mediated interference (RNAi), we show that PLP fibrils are required for interphase recruitment and proper mitotic assembly of the PCM matrix.",
author = "V. Mennella and B. Keszthelyi and Mcdonald, \{K. L.\} and B. Chhun and F. Kan and Rogers, \{G. C.\} and B. Huang and Agard, \{D. A.\}",
note = "Funding Information: We especially thank J. Sedat, L. Winoto and C. Weisiger for invaluable assistance with the OMX, S. Li for advice and help with the 3D alignment strategy, and D. Buster and K. Model for comments and editing on the manuscript. We also would like to thank T. Avidor-reiss (Harvard University, USA), J. Raff (University of Oxford, UK), M. Bettencourt-Dias (Instituto Gulbenkian de Ciencia, Portugal), M. Gatti (Universit{\'a} La Sapienza di Roma, Italy), T. Megraw (Florida State University, USA), M. Takahashi (Teikyo Heisei University, Japan), Y. Zheng (Carnegie Institution of Washington) and Howard Hughes Medical Institute, T. Davis (University of Washington) and T. Kaufman (University of Indiana, USA) for generously sharing their antibodies. This work was financially supported by HHMI and NIH grant GM310627. B.H. is a recipient of the Searle Scholarship and the Packard Fellowship for Science and Engineering.",
year = "2012",
month = nov,
doi = "10.1038/ncb2597",
language = "English (US)",
volume = "14",
pages = "1159--1168",
journal = "Nature Cell Biology",
issn = "1465-7392",
publisher = "Nature Research",
number = "11",
}