TY - JOUR
T1 - Studies of an androgen-binding protein knockout corroborate a role for salivary ABP in mouse communication
AU - Chung, Amanda G.
AU - Belone, Phillip M.
AU - Bímová, Barbora Vošlajerová
AU - Karn, Robert C.
AU - Laukaitis, Christina M.
N1 - Funding Information:
The authors thank Corina Mauss, Anastasia Miramontes, Georgina Linton-Smart, and a number of summer students who assisted in performing mouse husbandry and genotyping; Petr Šolc at the Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic who kindly provided C57BL/6 mice for behavioral testing; and Rob Beynon and Philip Brownridge for quantifying the data produced by the University of Arizona (UA) Proteomics Core. They gratefully acknowledge the Genetically Engineered Mouse Modeling Core, BIO5 Institute, UA who produced the knockout mouse with funding from the National Cancer Institute at the National Institutes of Health (NCI/ NIH) grant #P30 CA23074 and from the Department of Medicine, UA. A.G.C. was supported in part by grant #52005889 to UA from the Howard Hughes Medical Institute. P.M.B. was funded by NCI/NIH grant #CA143924 to the Partnership for Native American Cancer Prevention. B.V.B. was supported in part by grant #15-13265S from the Czech Science Foundation. C.M.L. received funding from NCI/NIH grant #PCA23074 and from the Vice President for Health Sciences, UA. Mass spectrometry and proteomics data were acquired by the Arizona Proteomics Consortium supported by National Institute of Environmental Health Sciences grant #ES06694 to the Southwest Environmental Health Sciences Center, NIH/NCI grant # CA023074 to the UA Cancer Center, and by the BIO5 Institute of UA. The Thermo Fisher LTQ Orbitrap Velos mass spectrometer was provided by grant #1S10 RR028868-01 from the NIH National Center for Research Resources.
Publisher Copyright:
© 2017 by the Genetics Society of America.
PY - 2017/4
Y1 - 2017/4
N2 - The house mouse Androgen-binding protein (Abp) gene family is comprised of 64 paralogs, 30 Abpa and 34 Abpbg, encoding the alpha (ABPA) and beta-gamma (ABPBG) protein subunits that are disulfide-bridged to form dimers in secretions. Only 14 Abp genes are expressed in distinct patterns in the lacrimal (11) and submandibular glands (3). We created a knockout mouse line lacking two of the three genes expressed in submandibular glands, Abpa27 and Abpbg27, by replacing them with the neomycin resistance gene. The knockout genotype (-/-) showed no Abpa27 or Abpbg27 transcripts in submandibular gland complementary DNA (cDNA) libraries and there was a concomitant lack of protein expression of ABPA27 and ABPBG27 in the -/- genotype saliva, shown by elimination of these two proteins from the saliva proteome and the loss of cross-reactive material in the acinar cells of the submandibular glands. We also observed a decrease in BG26 protein in the -/- animals, suggesting monomer instability. Overall, we observed no major phenotypic changes in the -/- genotype, compared with their +/+ and +/- siblings raised in a laboratory setting, including normal growth curves, tissue histology, fecundity, and longevity. The only difference is that male and female C57BL/6 mice preferred saliva of the opposite sex containing ABP statistically significantly more than saliva of the opposite sex without ABP in a Y-maze test. These results show for the first time that mice can sense the presence of ABP between saliva targets with and without ABPs, and that they spend more time investigating the target containing ABP.
AB - The house mouse Androgen-binding protein (Abp) gene family is comprised of 64 paralogs, 30 Abpa and 34 Abpbg, encoding the alpha (ABPA) and beta-gamma (ABPBG) protein subunits that are disulfide-bridged to form dimers in secretions. Only 14 Abp genes are expressed in distinct patterns in the lacrimal (11) and submandibular glands (3). We created a knockout mouse line lacking two of the three genes expressed in submandibular glands, Abpa27 and Abpbg27, by replacing them with the neomycin resistance gene. The knockout genotype (-/-) showed no Abpa27 or Abpbg27 transcripts in submandibular gland complementary DNA (cDNA) libraries and there was a concomitant lack of protein expression of ABPA27 and ABPBG27 in the -/- genotype saliva, shown by elimination of these two proteins from the saliva proteome and the loss of cross-reactive material in the acinar cells of the submandibular glands. We also observed a decrease in BG26 protein in the -/- animals, suggesting monomer instability. Overall, we observed no major phenotypic changes in the -/- genotype, compared with their +/+ and +/- siblings raised in a laboratory setting, including normal growth curves, tissue histology, fecundity, and longevity. The only difference is that male and female C57BL/6 mice preferred saliva of the opposite sex containing ABP statistically significantly more than saliva of the opposite sex without ABP in a Y-maze test. These results show for the first time that mice can sense the presence of ABP between saliva targets with and without ABPs, and that they spend more time investigating the target containing ABP.
KW - Androgen-binding protein
KW - Knockout mouse
KW - Label-free quantitative proteomics
KW - Preference testing
KW - Sexual selection
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U2 - 10.1534/genetics.116.194571
DO - 10.1534/genetics.116.194571
M3 - Article
C2 - 28159752
AN - SCOPUS:85020371168
VL - 205
SP - 1517
EP - 1527
JO - Genetics
JF - Genetics
SN - 0016-6731
IS - 4
ER -