Structure and nucleotide sequence of a Drosophila melanogaster protein kinase C gene.

A. Rosenthal; L. Rhee; R. Yadegari; R. Paro; A. Ullrich; D. V. Goeddel

doi:10.1002/j.1460-2075.1987.tb04773.x

Structure and nucleotide sequence of a Drosophila melanogaster protein kinase C gene.

A. Rosenthal, L. Rhee, R. Yadegari, R. Paro, A. Ullrich, D. V. Goeddel

Research output: Contribution to journal › Article › peer-review

102 Scopus citations

Abstract

Genomic and cDNA clones encoding a Drosophila melanogaster protein kinase C (PKC) homologue were identified using a bovine PKC cDNA probe. The cDNA clones contain a single open reading frame that encodes a 639 amino acid, 75-kd protein having extensive homology with bovine, human and rat PKC and homology with the kinase domains of other serine, threonine and tyrosine kinases. The Drosophila PKC gene is localized to region 53E of chromosome 2. The gene spans approximately 20 kb and contains at least 14 exons. Messenger RNA for PKC could not be detected in 0-3 h Drosophila embryos. Adult flies contain three PKC transcripts of 4.3, 4.0 and 2.4 kb.

Original language	English (US)
Pages (from-to)	433-441
Number of pages	9
Journal	The EMBO journal
Volume	6
Issue number	2
DOIs	https://doi.org/10.1002/j.1460-2075.1987.tb04773.x
State	Published - Feb 1987

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Biochemistry, Genetics and Molecular Biology(all)
Immunology and Microbiology(all)

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10.1002/j.1460-2075.1987.tb04773.x

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abstract = "Genomic and cDNA clones encoding a Drosophila melanogaster protein kinase C (PKC) homologue were identified using a bovine PKC cDNA probe. The cDNA clones contain a single open reading frame that encodes a 639 amino acid, 75-kd protein having extensive homology with bovine, human and rat PKC and homology with the kinase domains of other serine, threonine and tyrosine kinases. The Drosophila PKC gene is localized to region 53E of chromosome 2. The gene spans approximately 20 kb and contains at least 14 exons. Messenger RNA for PKC could not be detected in 0-3 h Drosophila embryos. Adult flies contain three PKC transcripts of 4.3, 4.0 and 2.4 kb.",

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T1 - Structure and nucleotide sequence of a Drosophila melanogaster protein kinase C gene.

AU - Rosenthal, A.

AU - Rhee, L.

AU - Yadegari, R.

AU - Paro, R.

AU - Ullrich, A.

AU - Goeddel, D. V.

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AB - Genomic and cDNA clones encoding a Drosophila melanogaster protein kinase C (PKC) homologue were identified using a bovine PKC cDNA probe. The cDNA clones contain a single open reading frame that encodes a 639 amino acid, 75-kd protein having extensive homology with bovine, human and rat PKC and homology with the kinase domains of other serine, threonine and tyrosine kinases. The Drosophila PKC gene is localized to region 53E of chromosome 2. The gene spans approximately 20 kb and contains at least 14 exons. Messenger RNA for PKC could not be detected in 0-3 h Drosophila embryos. Adult flies contain three PKC transcripts of 4.3, 4.0 and 2.4 kb.

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Structure and nucleotide sequence of a Drosophila melanogaster protein kinase C gene.

Abstract

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