Stress profiling of longevity mutants identifies Afg3 as a mitochondrial determinant of cytoplasmic mRNA translation and aging

Joe R. Delaney, Umema Ahmed, Annie Chou, Sylvia Sim, Daniel Carr, Christopher J. Murakami, Jennifer Schleit, George L. Sutphin, Elroy H. An, Anthony Castanza, Marissa Fletcher, Sean Higgins, Monika Jelic, Shannon Klum, Brian Muller, Zhao J. Peng, Dilreet Rai, Vanessa Ros, Minnie Singh, Helen V. WendeBrian K. Kennedy, Matt Kaeberlein

Research output: Contribution to journalArticlepeer-review

57 Scopus citations

Abstract

Although environmental stress likely plays a significant role in promoting aging, the relationship remains poorly understood. To characterize this interaction in a more comprehensive manner, we examined the stress response profiles for 46 long-lived yeast mutant strains across four different stress conditions (oxidative, ER, DNA damage, and thermal), grouping genes based on their associated stress response profiles. Unexpectedly, cells lacking the mitochondrial AAA protease gene AFG3 clustered strongly with long-lived strains lacking cytosolic ribosomal proteins of the large subunit. Similar to these ribosomal protein mutants, afg3Δ cells show reduced cytoplasmic mRNA translation, enhanced resistance to tunicamycin that is independent of the ER unfolded protein response, and Sir2-independent but Gcn4-dependent lifespan extension. These data demonstrate an unexpected link between a mitochondrial protease, cytoplasmic mRNA translation, and aging.

Original languageEnglish (US)
Pages (from-to)156-166
Number of pages11
JournalAging Cell
Volume12
Issue number1
DOIs
StatePublished - Feb 2013
Externally publishedYes

Keywords

  • Aging
  • Epistasis
  • ER stress
  • Longevity
  • Mitochondria
  • Phenotype mapping
  • Replicative lifespan
  • Stress response
  • Translation
  • Yeast

ASJC Scopus subject areas

  • Aging
  • Cell Biology

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