Stimulation by glucocorticoids of myoblast growth at low cell densities

Vincent Guerriero; James R. Florini

doi:10.1016/0309-1651(78)90095-4

Stimulation by glucocorticoids of myoblast growth at low cell densities

Vincent Guerriero, James R. Florini

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Proliferation of rat myoblasts was stimulated by addition of 10^-7 M dexamethasone to cells plated at 25 cells/cm². In the presence of fetal calf serum and chick embryo extract, physiological levels of glucocorticoids caused a 3- to 7-fold increase in colony formation. In mass cultures, proliferation of myoblasts was also stimulated by dexamethasone, but fibroblast growth was inhibited. These opposite effects of glucocorticoids on myoblast and fibroblast growth suggest their use in cloning muscle cells from mixed cell populations.

Original language	English (US)
Pages (from-to)	441-446
Number of pages	6
Journal	Cell Biology International Reports
Volume	2
Issue number	5
DOIs	https://doi.org/10.1016/0309-1651(78)90095-4
State	Published - Sep 1978
Externally published	Yes

ASJC Scopus subject areas

Cell Biology

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10.1016/0309-1651(78)90095-4

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@article{37a65a6a94a24b64bfe6fd48e49db64c,

title = "Stimulation by glucocorticoids of myoblast growth at low cell densities",

abstract = "Proliferation of rat myoblasts was stimulated by addition of 10-7 M dexamethasone to cells plated at 25 cells/cm2. In the presence of fetal calf serum and chick embryo extract, physiological levels of glucocorticoids caused a 3- to 7-fold increase in colony formation. In mass cultures, proliferation of myoblasts was also stimulated by dexamethasone, but fibroblast growth was inhibited. These opposite effects of glucocorticoids on myoblast and fibroblast growth suggest their use in cloning muscle cells from mixed cell populations.",

author = "Vincent Guerriero and Florini, {James R.}",

note = "Funding Information: We thank Dr. V. J. Cristofalo of the Wistar Institute for helpful discussions during the course of this work. We are grateful to :sabe1 Mellon and Daina Ewton for careful technical assistance with< these experiments. This work was supported by a grant from the ?ius-cular Dystrophy Association and by grant HL 11551 from the Nationa?! Institutes of Health.",

year = "1978",

month = sep,

doi = "10.1016/0309-1651(78)90095-4",

language = "English (US)",

volume = "2",

pages = "441--446",

journal = "Cell Biology International Reports",

issn = "0309-1651",

publisher = "Wiley-Blackwell",

number = "5",

}

TY - JOUR

T1 - Stimulation by glucocorticoids of myoblast growth at low cell densities

AU - Guerriero, Vincent

AU - Florini, James R.

N1 - Funding Information: We thank Dr. V. J. Cristofalo of the Wistar Institute for helpful discussions during the course of this work. We are grateful to :sabe1 Mellon and Daina Ewton for careful technical assistance with< these experiments. This work was supported by a grant from the ?ius-cular Dystrophy Association and by grant HL 11551 from the Nationa?! Institutes of Health.

PY - 1978/9

Y1 - 1978/9

N2 - Proliferation of rat myoblasts was stimulated by addition of 10-7 M dexamethasone to cells plated at 25 cells/cm2. In the presence of fetal calf serum and chick embryo extract, physiological levels of glucocorticoids caused a 3- to 7-fold increase in colony formation. In mass cultures, proliferation of myoblasts was also stimulated by dexamethasone, but fibroblast growth was inhibited. These opposite effects of glucocorticoids on myoblast and fibroblast growth suggest their use in cloning muscle cells from mixed cell populations.

AB - Proliferation of rat myoblasts was stimulated by addition of 10-7 M dexamethasone to cells plated at 25 cells/cm2. In the presence of fetal calf serum and chick embryo extract, physiological levels of glucocorticoids caused a 3- to 7-fold increase in colony formation. In mass cultures, proliferation of myoblasts was also stimulated by dexamethasone, but fibroblast growth was inhibited. These opposite effects of glucocorticoids on myoblast and fibroblast growth suggest their use in cloning muscle cells from mixed cell populations.

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EP - 446

JO - Cell Biology International Reports

JF - Cell Biology International Reports

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ER -

Stimulation by glucocorticoids of myoblast growth at low cell densities

Abstract

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