Stigma receptors control intraspecies and interspecies barriers in Brassicaceae

Jiabao Huang, Lin Yang, Liu Yang, Xiaoyu Wu, Xiaoshuang Cui, Lili Zhang, Jiyun Hui, Yumei Zhao, Hongmin Yang, Shangjia Liu, Quanling Xu, Maoxuan Pang, Xinping Guo, Yunyun Cao, Yu Chen, Xinru Ren, Jinzhi Lv, Jianqiang Yu, Junyi Ding, Gang XuNian Wang, Xiaochun Wei, Qinghui Lin, Yuxiang Yuan, Xiaowei Zhang, Chaozhi Ma, Cheng Dai, Pengwei Wang, Yongchao Wang, Fei Cheng, Weiqing Zeng, Ravishankar Palanivelu, Hen Ming Wu, Xiansheng Zhang, Alice Y. Cheung, Qiaohong Duan

Research output: Contribution to journalArticlepeer-review

48 Scopus citations

Abstract

Flowering plants have evolved numerous intraspecific and interspecific prezygotic reproductive barriers to prevent production of unfavourable offspring1. Within a species, self-incompatibility (SI) is a widely utilized mechanism that rejects self-pollen2,3 to avoid inbreeding depression. Interspecific barriers restrain breeding between species and often follow the SI × self-compatible (SC) rule, that is, interspecific pollen is unilaterally incompatible (UI) on SI pistils but unilaterally compatible (UC) on SC pistils1,4–6. The molecular mechanisms underlying SI, UI, SC and UC and their interconnections in the Brassicaceae remain unclear. Here we demonstrate that the SI pollen determinant S-locus cysteine-rich protein/S-locus protein 11 (SCR/SP11)2,3 or a signal from UI pollen binds to the SI female determinant S-locus receptor kinase (SRK)2,3, recruits FERONIA (FER)7–9 and activates FER-mediated reactive oxygen species production in SI stigmas10,11 to reject incompatible pollen. For compatible responses, diverged pollen coat protein B-class12–14 from SC and UC pollen differentially trigger nitric oxide, nitrosate FER to suppress reactive oxygen species in SC stigmas to facilitate pollen growth in an intraspecies-preferential manner, maintaining species integrity. Our results show that SRK and FER integrate mechanisms underlying intraspecific and interspecific barriers and offer paths to achieve distant breeding in Brassicaceae crops.

Original languageEnglish (US)
Pages (from-to)303-308
Number of pages6
JournalNature
Volume614
Issue number7947
DOIs
StatePublished - Feb 9 2023

ASJC Scopus subject areas

  • General

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