TY - JOUR
T1 - Stigma receptors control intraspecies and interspecies barriers in Brassicaceae
AU - Huang, Jiabao
AU - Yang, Lin
AU - Yang, Liu
AU - Wu, Xiaoyu
AU - Cui, Xiaoshuang
AU - Zhang, Lili
AU - Hui, Jiyun
AU - Zhao, Yumei
AU - Yang, Hongmin
AU - Liu, Shangjia
AU - Xu, Quanling
AU - Pang, Maoxuan
AU - Guo, Xinping
AU - Cao, Yunyun
AU - Chen, Yu
AU - Ren, Xinru
AU - Lv, Jinzhi
AU - Yu, Jianqiang
AU - Ding, Junyi
AU - Xu, Gang
AU - Wang, Nian
AU - Wei, Xiaochun
AU - Lin, Qinghui
AU - Yuan, Yuxiang
AU - Zhang, Xiaowei
AU - Ma, Chaozhi
AU - Dai, Cheng
AU - Wang, Pengwei
AU - Wang, Yongchao
AU - Cheng, Fei
AU - Zeng, Weiqing
AU - Palanivelu, Ravishankar
AU - Wu, Hen Ming
AU - Zhang, Xiansheng
AU - Cheung, Alice Y.
AU - Duan, Qiaohong
N1 - Publisher Copyright:
© 2023, The Author(s).
PY - 2023/2/9
Y1 - 2023/2/9
N2 - Flowering plants have evolved numerous intraspecific and interspecific prezygotic reproductive barriers to prevent production of unfavourable offspring1. Within a species, self-incompatibility (SI) is a widely utilized mechanism that rejects self-pollen2,3 to avoid inbreeding depression. Interspecific barriers restrain breeding between species and often follow the SI × self-compatible (SC) rule, that is, interspecific pollen is unilaterally incompatible (UI) on SI pistils but unilaterally compatible (UC) on SC pistils1,4–6. The molecular mechanisms underlying SI, UI, SC and UC and their interconnections in the Brassicaceae remain unclear. Here we demonstrate that the SI pollen determinant S-locus cysteine-rich protein/S-locus protein 11 (SCR/SP11)2,3 or a signal from UI pollen binds to the SI female determinant S-locus receptor kinase (SRK)2,3, recruits FERONIA (FER)7–9 and activates FER-mediated reactive oxygen species production in SI stigmas10,11 to reject incompatible pollen. For compatible responses, diverged pollen coat protein B-class12–14 from SC and UC pollen differentially trigger nitric oxide, nitrosate FER to suppress reactive oxygen species in SC stigmas to facilitate pollen growth in an intraspecies-preferential manner, maintaining species integrity. Our results show that SRK and FER integrate mechanisms underlying intraspecific and interspecific barriers and offer paths to achieve distant breeding in Brassicaceae crops.
AB - Flowering plants have evolved numerous intraspecific and interspecific prezygotic reproductive barriers to prevent production of unfavourable offspring1. Within a species, self-incompatibility (SI) is a widely utilized mechanism that rejects self-pollen2,3 to avoid inbreeding depression. Interspecific barriers restrain breeding between species and often follow the SI × self-compatible (SC) rule, that is, interspecific pollen is unilaterally incompatible (UI) on SI pistils but unilaterally compatible (UC) on SC pistils1,4–6. The molecular mechanisms underlying SI, UI, SC and UC and their interconnections in the Brassicaceae remain unclear. Here we demonstrate that the SI pollen determinant S-locus cysteine-rich protein/S-locus protein 11 (SCR/SP11)2,3 or a signal from UI pollen binds to the SI female determinant S-locus receptor kinase (SRK)2,3, recruits FERONIA (FER)7–9 and activates FER-mediated reactive oxygen species production in SI stigmas10,11 to reject incompatible pollen. For compatible responses, diverged pollen coat protein B-class12–14 from SC and UC pollen differentially trigger nitric oxide, nitrosate FER to suppress reactive oxygen species in SC stigmas to facilitate pollen growth in an intraspecies-preferential manner, maintaining species integrity. Our results show that SRK and FER integrate mechanisms underlying intraspecific and interspecific barriers and offer paths to achieve distant breeding in Brassicaceae crops.
UR - http://www.scopus.com/inward/record.url?scp=85146880823&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85146880823&partnerID=8YFLogxK
U2 - 10.1038/s41586-022-05640-x
DO - 10.1038/s41586-022-05640-x
M3 - Article
C2 - 36697825
AN - SCOPUS:85146880823
SN - 0028-0836
VL - 614
SP - 303
EP - 308
JO - Nature
JF - Nature
IS - 7947
ER -