Steps toward mapping the human vasculature by phage display

Wadih Arap, Mikhail G. Kolonin, Martin Trepel, Johanna Lahdenranta, Marina Cardó-Vila, Ricardo J. Giordano, Paul J. Mintz, Peter U. Ardelt, Virginia J. Yao, Claudia I. Vidal, Limor Chen, Anne Flamm, Heli Valtanen, Lisa M. Weavind, Marshall E. Hicks, Raphael E. Pollock, Gregory H. Botz, Corazon D. Bucana, Erkki Koivunen, Dolores CahillPatricia Troncoso, Keith A. Baggerly, Rebecca D. Pentz, Kim Anh Do, Christopher J. Logothetis, Renata Pasqualini

Research output: Contribution to journalArticlepeer-review

493 Scopus citations

Abstract

The molecular diversity of receptors in human blood vessels remains largely unexplored. We developed a selection method in which peptides that home to specific vascular beds are identified after administration of a peptide library. Here we report the first in vivo screening of a peptide library in a patient. We surveyed 47, 160 motifs that localized to different organs. This large-scale screening indicates that the tissue distribution of circulating peptides is nonrandom. High-throughput analysis of the motifs revealed similarities to ligands for differentially expressed cell-surface proteins, and a candidate ligand-receptor pair was validated. These data represent a step toward the construction of a molecular map of human vasculature and may have broad implications for the development of targeted therapies.

Original languageEnglish (US)
Pages (from-to)121-127
Number of pages7
JournalNature Medicine
Volume8
Issue number2
DOIs
StatePublished - Feb 2002
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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