Stability of lipid films formed on λ-aminopropyl monolayers

Todd W. McBee; S. Scott Saavedra

doi:10.1021/la047646f

Stability of lipid films formed on λ-aminopropyl monolayers

Todd W. McBee, S. Scott Saavedra

Chemistry and Biochemistry

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

The stability of supported lipid membranes (SLMs) deposited on planar substrates derivatized with (λ-aminopropyl)silane (GAPS) was examined. Ellipsometry, fluorescence microscopy, and atomic force microscopy were used to characterize SLMs exposed to repeated drying and rehydration. Vesicle fusion on GAPS-coated substrates produced SLMs with a thickness significantly greater than that of a single lipid bilayer. Exposure to even one cycle of drying/rehydration significantly decreased the thickness of a SLM on GAPS, and repeated drying/rehydration resulted in near quantitative lipid desorption. Thus SLMs on GAPS do not appear to be significantly more stable than the single bilayer SLM that is formed on bare glass or SiO ₂ under equivalent conditions.

Original language	English (US)
Pages (from-to)	3396-3399
Number of pages	4
Journal	Langmuir
Volume	21
Issue number	8
DOIs	https://doi.org/10.1021/la047646f
State	Published - Apr 12 2005

ASJC Scopus subject areas

General Materials Science
Condensed Matter Physics
Surfaces and Interfaces
Spectroscopy
Electrochemistry

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10.1021/la047646f

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abstract = "The stability of supported lipid membranes (SLMs) deposited on planar substrates derivatized with (λ-aminopropyl)silane (GAPS) was examined. Ellipsometry, fluorescence microscopy, and atomic force microscopy were used to characterize SLMs exposed to repeated drying and rehydration. Vesicle fusion on GAPS-coated substrates produced SLMs with a thickness significantly greater than that of a single lipid bilayer. Exposure to even one cycle of drying/rehydration significantly decreased the thickness of a SLM on GAPS, and repeated drying/rehydration resulted in near quantitative lipid desorption. Thus SLMs on GAPS do not appear to be significantly more stable than the single bilayer SLM that is formed on bare glass or SiO 2 under equivalent conditions.",

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N2 - The stability of supported lipid membranes (SLMs) deposited on planar substrates derivatized with (λ-aminopropyl)silane (GAPS) was examined. Ellipsometry, fluorescence microscopy, and atomic force microscopy were used to characterize SLMs exposed to repeated drying and rehydration. Vesicle fusion on GAPS-coated substrates produced SLMs with a thickness significantly greater than that of a single lipid bilayer. Exposure to even one cycle of drying/rehydration significantly decreased the thickness of a SLM on GAPS, and repeated drying/rehydration resulted in near quantitative lipid desorption. Thus SLMs on GAPS do not appear to be significantly more stable than the single bilayer SLM that is formed on bare glass or SiO 2 under equivalent conditions.

AB - The stability of supported lipid membranes (SLMs) deposited on planar substrates derivatized with (λ-aminopropyl)silane (GAPS) was examined. Ellipsometry, fluorescence microscopy, and atomic force microscopy were used to characterize SLMs exposed to repeated drying and rehydration. Vesicle fusion on GAPS-coated substrates produced SLMs with a thickness significantly greater than that of a single lipid bilayer. Exposure to even one cycle of drying/rehydration significantly decreased the thickness of a SLM on GAPS, and repeated drying/rehydration resulted in near quantitative lipid desorption. Thus SLMs on GAPS do not appear to be significantly more stable than the single bilayer SLM that is formed on bare glass or SiO 2 under equivalent conditions.

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Stability of lipid films formed on λ-aminopropyl monolayers

Abstract

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