Spider, bacterial and fungal phospholipase D toxins make cyclic phosphate products

Daniel M. Lajoie; Matthew H.J. Cordes

doi:10.1016/j.toxicon.2015.10.008

Spider, bacterial and fungal phospholipase D toxins make cyclic phosphate products

Daniel M. Lajoie, Matthew H.J. Cordes

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

Phospholipase D (PLD) toxins from sicariid spiders, which cause disease in mammals, were recently found to convert their primary substrates, sphingomyelin and lysophosphatidylcholine, to cyclic phospholipids. Here we show that two PLD toxins from pathogenic actinobacteria and ascomycete fungi, which share distant homology with the spider toxins, also generate cyclic phospholipids. This shared function supports divergent evolution of the PLD toxins from a common ancestor and suggests the importance of cyclic phospholipids in pathogenicity.

Original language	English (US)
Pages (from-to)	176-180
Number of pages	5
Journal	Toxicon
Volume	108
DOIs	https://doi.org/10.1016/j.toxicon.2015.10.008
State	Published - Dec 15 2015

Keywords

Arcanobacterium haemolyticum
Coccidioides posadasii
P-NMR
Phospholipase D
Toxin evolution

ASJC Scopus subject areas

Toxicology

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10.1016/j.toxicon.2015.10.008

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title = "Spider, bacterial and fungal phospholipase D toxins make cyclic phosphate products",

abstract = "Phospholipase D (PLD) toxins from sicariid spiders, which cause disease in mammals, were recently found to convert their primary substrates, sphingomyelin and lysophosphatidylcholine, to cyclic phospholipids. Here we show that two PLD toxins from pathogenic actinobacteria and ascomycete fungi, which share distant homology with the spider toxins, also generate cyclic phospholipids. This shared function supports divergent evolution of the PLD toxins from a common ancestor and suggests the importance of cyclic phospholipids in pathogenicity.",

keywords = "Arcanobacterium haemolyticum, Coccidioides posadasii, P-NMR, Phospholipase D, Toxin evolution",

author = "Lajoie, {Daniel M.} and Cordes, {Matthew H.J.}",

note = "Funding Information: We thank David McGee (LSU-HSC Shreveport), and Helen Jost (University of Arizona) for the gift of a plasmid encoding the Arcanobacterium haemolyticum PLD, and Greta Binford and Pamela Zobel-Thropp for supplying the Loxosceles arizonica clones. This work was supported by a pilot project grant from the Bio5 Institute in Tucson, Arizona. Publisher Copyright: {\textcopyright} 2015 Elsevier Ltd. All rights reserved.",

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doi = "10.1016/j.toxicon.2015.10.008",

language = "English (US)",

volume = "108",

pages = "176--180",

journal = "Toxicon",

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T1 - Spider, bacterial and fungal phospholipase D toxins make cyclic phosphate products

AU - Lajoie, Daniel M.

AU - Cordes, Matthew H.J.

N1 - Funding Information: We thank David McGee (LSU-HSC Shreveport), and Helen Jost (University of Arizona) for the gift of a plasmid encoding the Arcanobacterium haemolyticum PLD, and Greta Binford and Pamela Zobel-Thropp for supplying the Loxosceles arizonica clones. This work was supported by a pilot project grant from the Bio5 Institute in Tucson, Arizona. Publisher Copyright: © 2015 Elsevier Ltd. All rights reserved.

PY - 2015/12/15

Y1 - 2015/12/15

N2 - Phospholipase D (PLD) toxins from sicariid spiders, which cause disease in mammals, were recently found to convert their primary substrates, sphingomyelin and lysophosphatidylcholine, to cyclic phospholipids. Here we show that two PLD toxins from pathogenic actinobacteria and ascomycete fungi, which share distant homology with the spider toxins, also generate cyclic phospholipids. This shared function supports divergent evolution of the PLD toxins from a common ancestor and suggests the importance of cyclic phospholipids in pathogenicity.

AB - Phospholipase D (PLD) toxins from sicariid spiders, which cause disease in mammals, were recently found to convert their primary substrates, sphingomyelin and lysophosphatidylcholine, to cyclic phospholipids. Here we show that two PLD toxins from pathogenic actinobacteria and ascomycete fungi, which share distant homology with the spider toxins, also generate cyclic phospholipids. This shared function supports divergent evolution of the PLD toxins from a common ancestor and suggests the importance of cyclic phospholipids in pathogenicity.

KW - Arcanobacterium haemolyticum

KW - Coccidioides posadasii

KW - P-NMR

KW - Phospholipase D

KW - Toxin evolution

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DO - 10.1016/j.toxicon.2015.10.008

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VL - 108

SP - 176

EP - 180

JO - Toxicon

JF - Toxicon

ER -

Spider, bacterial and fungal phospholipase D toxins make cyclic phosphate products

Abstract

Keywords

ASJC Scopus subject areas

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