Sphingolipid analysis by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS)

Jacek Bielawski, Jason S. Pierce, Justin Snider, Barbara Rembiesa, Zdzislaw M. Szulc, Alicja Bielawska

Research output: Chapter in Book/Report/Conference proceedingChapter

94 Scopus citations

Abstract

Sphingolipid (SPL) metabolism (Fig. 1) serves a key role in the complex mechanisms regulating cellular stress responses to environment. Several SPL metabolites, especially ceramide (Cer), sphingosine (Sph) and sphingosine1-phosphate (S1P) act as key bioactive molecules governing cell growth and programmed cell death (Fig. 2). Perturbations in sphingolipids of one type may enhance or interfere with the action of another. To monitor changes in SPL composition therefore, reliable analytical methods are necessary. Here we present the liquid chromatography tandem mass spectrometry (LC-MS/MS) approach for simultaneous qualitative and quantitative monitoring of SPL components (classes and molecular species) in biological material as an effective tool to study sphingolipid signaling events. The LC-MS/MS methodology is the only available technique that provides high specificity and sensitivity, along with a wealth of structural identification information

Original languageEnglish (US)
Title of host publicationSphingolipids as Signaling and Regulatory Molecules
EditorsCharles Chalfant, Maurizio Del Poeta
Pages46-59
Number of pages14
DOIs
StatePublished - 2010
Externally publishedYes

Publication series

NameAdvances in Experimental Medicine and Biology
Volume688
ISSN (Print)0065-2598

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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