Spectral light quality affects protein profile of Synechococcus sp. PCC 7942: A comparative 2-dimensional gel electrophoresis (2-DGE) analysis

Ritu Pandey, Sanjay Chauhan, Gauri S. Singhal, R. K. Kale

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

We report here a comparative analysis of the effect of blue (450 nm), red (660 nm), and white light (400-700 nm) on the protein profile of cyanobacteria Synechococcus sp. PCC 7942. In vivo labeling of cells with [35S] methionine and their subsequent analysis by two-dimensional gel electrophoresis (2-DGE) showed that eight polypeptides were unique to dark adapted cells, ten were blue light specific, and four were specifically induced in red light. The results show that Synechococcus sp. respond to various light treatments rapidly and synthesize new polypeptides in dark and blue/red light.

Original languageEnglish (US)
Pages (from-to)297-301
Number of pages5
JournalCurrent Microbiology
Volume40
Issue number5
DOIs
StatePublished - 2000
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

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