Sp1 Affinity for GC-Rich Elements Correlates with Ornithine Decarboxylase Promoter Activity

Rameez Alasadi, Eugene C. Yi, Juanita L. Merchant

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

The highly conserved ornithine decarboxylase (E.C.4.1.1.17) promoter contains multiple binding sites for Sp1 within the first 400 bp of the cap site. Therefore the ability of individual Sp1 elements to confer transactivation alone or in combination was tested. We show that different Sp1 sites vary in their affinity for Sp1 and that increasing affinity correlates with enhancer activity. In addition, several adjacent Sp1 sites synergistically enhanced promoter activity. Thus, the strength of promoter transactivation correlated with both the number of GC-rich elements and their affinity for Sp1 protein.

Original languageEnglish (US)
Pages (from-to)324-330
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume214
Issue number2
DOIs
StatePublished - 1995
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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