TY - JOUR
T1 - Sp1 Affinity for GC-Rich Elements Correlates with Ornithine Decarboxylase Promoter Activity
AU - Alasadi, Rameez
AU - Yi, Eugene C.
AU - Merchant, Juanita L.
PY - 1995
Y1 - 1995
N2 - The highly conserved ornithine decarboxylase (E.C.4.1.1.17) promoter contains multiple binding sites for Sp1 within the first 400 bp of the cap site. Therefore the ability of individual Sp1 elements to confer transactivation alone or in combination was tested. We show that different Sp1 sites vary in their affinity for Sp1 and that increasing affinity correlates with enhancer activity. In addition, several adjacent Sp1 sites synergistically enhanced promoter activity. Thus, the strength of promoter transactivation correlated with both the number of GC-rich elements and their affinity for Sp1 protein.
AB - The highly conserved ornithine decarboxylase (E.C.4.1.1.17) promoter contains multiple binding sites for Sp1 within the first 400 bp of the cap site. Therefore the ability of individual Sp1 elements to confer transactivation alone or in combination was tested. We show that different Sp1 sites vary in their affinity for Sp1 and that increasing affinity correlates with enhancer activity. In addition, several adjacent Sp1 sites synergistically enhanced promoter activity. Thus, the strength of promoter transactivation correlated with both the number of GC-rich elements and their affinity for Sp1 protein.
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U2 - 10.1006/bbrc.1995.2291
DO - 10.1006/bbrc.1995.2291
M3 - Article
C2 - 7677738
AN - SCOPUS:0029098465
SN - 0006-291X
VL - 214
SP - 324
EP - 330
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -