TY - JOUR
T1 - Sorptive fractionation of organic matter and formation of organo-hydroxy-aluminum complexes during litter biodegradation in the presence of gibbsite
AU - Heckman, K.
AU - Grandy, A. S.
AU - Gao, X.
AU - Keiluweit, M.
AU - Wickings, K.
AU - Carpenter, K.
AU - Chorover, J.
AU - Rasmussen, C.
N1 - Funding Information:
This work was supported by the National Science Foundation ( DEB 0543130 and 0918718 , and EAR 0724958 ). A portion of this work was performed under the auspices of the U.S. Department of Energy by Lawrence Livermore National Laboratory under Contract DE-AC52-07NA27344, LLNL-JRNL-581333. Forest floor samples used in this study were collected from the Santa Catalina Mountains Critical Zone Observatory (SCM-CZO, NSF grant EAR 0724958 ). The authors wish to thank Dr. S. Mercer Meding (through the support of NSF EAR 0929850 ) for his dedicated assistance throughout this project, and Dr. C Swanston for his unflagging moral and intellectual support. The authors gratefully acknowledge A.L.D. Kilcoyne for his help and support at ALS beamlines 5.3.2.2 and 5.3.2.1 of the Advanced Light Source. The Advanced Light Source is supported by the Director, Office of Science, Office of Basic Energy Sciences, of the U.S. DOE under Contract No. DE-AC02-05CH11231. The authors extend their sincere thanks to the associate editor, Dr. Marc Norman, and two anonymous reviewers who substantially improved the quality of this manuscript.
PY - 2013/11/15
Y1 - 2013/11/15
N2 - Solid and aqueous phase Al species are recognized to affect organic matter (OM) stabilization in forest soils. However, little is known about the dynamics of formation, composition and dissolution of organo-Al hydroxide complexes in microbially-active soil systems, where plant litter is subject to microbial decomposition in close proximity to mineral weathering reactions. We incubated gibbsite-quartz mineral mixtures in the presence of forest floor material inoculated with a native microbial consortium for periods of 5, 60 and 154days. At each time step, samples were density separated into light (<1.6gcm-3), intermediate (1.6-2.0gcm-3), and heavy (>2.0gcm-3) fractions. The light fraction was mainly comprised of particulate organic matter, while the intermediate and heavy density fractions contained moderate and large amounts of Al-minerals, respectively. Multi-method interrogation of the fractions indicated the intermediate and heavy fractions differed both in mineral structure and organic compound composition. X-ray diffraction analysis and SEM/EDS of the mineral component of the intermediate fractions indicated some alteration of the original gibbsite structure into less crystalline Al hydroxide and possibly proto-imogolite species, whereas alteration of the gibbsite structure was not evident in the heavy fraction. DRIFT, Py-GC/MS and STXM/NEXAFS results all showed that intermediate fractions were composed mostly of lignin-derived compounds, phenolics, and polysaccharides. Heavy fraction organics were dominated by polysaccharides, and were enriched in proteins, N-bearing compounds, and lipids. The source of organics appeared to differ between the intermediate and heavy fractions. Heavy fractions were enriched in 13C with lower C/N ratios relative to intermediate fractions, suggesting a microbial origin. The observed differential fractionation of organics among hydroxy-Al mineral types suggests that microbial activity superimposed with abiotic mineral-surface-mediated fractionation leads to strong density differentiation of organo-mineral complex composition even over the short time scales probed in these incubation experiments. The data highlight the strong interdependency of mineral transformation, microbial community activity, and organic matter stabilization during biodegradation.
AB - Solid and aqueous phase Al species are recognized to affect organic matter (OM) stabilization in forest soils. However, little is known about the dynamics of formation, composition and dissolution of organo-Al hydroxide complexes in microbially-active soil systems, where plant litter is subject to microbial decomposition in close proximity to mineral weathering reactions. We incubated gibbsite-quartz mineral mixtures in the presence of forest floor material inoculated with a native microbial consortium for periods of 5, 60 and 154days. At each time step, samples were density separated into light (<1.6gcm-3), intermediate (1.6-2.0gcm-3), and heavy (>2.0gcm-3) fractions. The light fraction was mainly comprised of particulate organic matter, while the intermediate and heavy density fractions contained moderate and large amounts of Al-minerals, respectively. Multi-method interrogation of the fractions indicated the intermediate and heavy fractions differed both in mineral structure and organic compound composition. X-ray diffraction analysis and SEM/EDS of the mineral component of the intermediate fractions indicated some alteration of the original gibbsite structure into less crystalline Al hydroxide and possibly proto-imogolite species, whereas alteration of the gibbsite structure was not evident in the heavy fraction. DRIFT, Py-GC/MS and STXM/NEXAFS results all showed that intermediate fractions were composed mostly of lignin-derived compounds, phenolics, and polysaccharides. Heavy fraction organics were dominated by polysaccharides, and were enriched in proteins, N-bearing compounds, and lipids. The source of organics appeared to differ between the intermediate and heavy fractions. Heavy fractions were enriched in 13C with lower C/N ratios relative to intermediate fractions, suggesting a microbial origin. The observed differential fractionation of organics among hydroxy-Al mineral types suggests that microbial activity superimposed with abiotic mineral-surface-mediated fractionation leads to strong density differentiation of organo-mineral complex composition even over the short time scales probed in these incubation experiments. The data highlight the strong interdependency of mineral transformation, microbial community activity, and organic matter stabilization during biodegradation.
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U2 - 10.1016/j.gca.2013.07.043
DO - 10.1016/j.gca.2013.07.043
M3 - Article
AN - SCOPUS:84884147607
SN - 0016-7037
VL - 121
SP - 667
EP - 683
JO - Geochimica et Cosmochimica Acta
JF - Geochimica et Cosmochimica Acta
ER -