TY - JOUR
T1 - Soluble N-ethylmaleimide-sensitive Factor Attachment Receptor (SNARE) Protein Involved in the Remission of Depression by Acupuncture in Rats
AU - Fan, Ling
AU - Chen, Zhao
AU - Fu, Wenbin
AU - Xu, Nenggui
AU - Liu, Jianhua
AU - Lu, Aiping
AU - Li, Ziping
AU - Su, Shengyong
AU - Wu, Taixiang
AU - Ou, Aihua
N1 - Funding Information:
The authors express their deepest appreciation to the staff of the Scientific Research Department of Guangdong Hospital of Traditional Chinese Medicine for its technical support and to the staff of the Division of Epidemiology and Biostatistics, Mel and Enid Zuckerman College of Public Health, University of Arizona, for its analysis of the data. This research was supported by (1) the Youth Fund Project of the Natural Science Foundation of China: Antidepressant mechanism of acupuncture based on SNARE proteins modulating the release of glutamic acid in presynaptic neurons (81303041); (2) A class general financial grant from the China Postdoctoral Science Foundation: Mechanism research of acupuncture on hippocampal tripartite glutamatergic synapses of depressive disorder rats (2012M511784); (3) Science Foundation of Outstanding Young Innovative Personnel, Department of Education, Guangdong Province: Clinical research of acupuncture in the treatment of mild to moderate depression (2012LYM_0043); (4) Special research foundation of the new teacher category for the doctoral program of higher school sponsored by the National Ministry of Education: The moderating effects of acupuncture for hippocampal tripartite glutamatergic synapses of depressive disorder rats (20124425120005); (5) Special financial grant from the China Postdoctoral Science Foundation: The modulating effects of acupuncture for glutamic acid in hippocampal presynaptic neurons of depressive disorder rats (2013T60793); and (6) Science Foundation of the Postdoctoral Researchers, Guangzhou University of Chinese Medicine, from Guangdong Provincial Department of Human Resources and Social Security Fund: Clinical study on acupuncture and moxibustion for treatment of depression based on patient reported outcome indicators (BBK429122K19).
Publisher Copyright:
© 2016
PY - 2016/10/1
Y1 - 2016/10/1
N2 - This study aims to investigate the molecular mechanisms of acupuncture in the remission of depression. A depressive disorder model was induced by exposing Sprague–Dawley rats to chronic unpredictable stress. The rats were divided into five groups: healthy (blank group) and stressed rats (model group), and stressed rats treated with acupuncture (acupuncture group), riluzole (riluzole group), acupuncture combined with botulinum toxin A (BTX-A) injection (acupuncture + BTX-A group) or riluzole combined with BTX-A injection (riluzole + BTX-A group). Behavioral analysis showed significant differences in sucrose consumption, weight, and horizontal or vertical movements between the model and both the riluzole and acupuncture groups. No obvious differences between the riluzole + BTX-A and acupuncture + BTX-A groups were found. Moreover, no significance differences in glutamate content in the hippocampus were found among the riluzole + BTX-A, acupuncture + BTX-A and model groups (p > 0.05). Western blots and reverse transcription polymerase chain reactions were employed to detect protein and mRNA expressions of VGLUT2, SNAP25, VAMP1, VAMP2, VAMP7, and syntaxin1; no obvious differences among the riluzole + BTX-A, acupuncture + BTX-A and model groups were found. These data suggest that soluble N-ethylmaleimide-sensitive factor attachment receptor proteins are involved in the remission of depression in rats treated with acupuncture.
AB - This study aims to investigate the molecular mechanisms of acupuncture in the remission of depression. A depressive disorder model was induced by exposing Sprague–Dawley rats to chronic unpredictable stress. The rats were divided into five groups: healthy (blank group) and stressed rats (model group), and stressed rats treated with acupuncture (acupuncture group), riluzole (riluzole group), acupuncture combined with botulinum toxin A (BTX-A) injection (acupuncture + BTX-A group) or riluzole combined with BTX-A injection (riluzole + BTX-A group). Behavioral analysis showed significant differences in sucrose consumption, weight, and horizontal or vertical movements between the model and both the riluzole and acupuncture groups. No obvious differences between the riluzole + BTX-A and acupuncture + BTX-A groups were found. Moreover, no significance differences in glutamate content in the hippocampus were found among the riluzole + BTX-A, acupuncture + BTX-A and model groups (p > 0.05). Western blots and reverse transcription polymerase chain reactions were employed to detect protein and mRNA expressions of VGLUT2, SNAP25, VAMP1, VAMP2, VAMP7, and syntaxin1; no obvious differences among the riluzole + BTX-A, acupuncture + BTX-A and model groups were found. These data suggest that soluble N-ethylmaleimide-sensitive factor attachment receptor proteins are involved in the remission of depression in rats treated with acupuncture.
KW - acupuncture
KW - antidepressant
KW - botulinum toxin A
KW - riluzole
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U2 - 10.1016/j.jams.2016.04.002
DO - 10.1016/j.jams.2016.04.002
M3 - Article
C2 - 27776762
AN - SCOPUS:84973618667
SN - 2005-2901
VL - 9
SP - 242
EP - 249
JO - JAMS Journal of Acupuncture and Meridian Studies
JF - JAMS Journal of Acupuncture and Meridian Studies
IS - 5
ER -