TY - JOUR
T1 - Solid tumor inhibitory and other constituents of Casimiroa tetrameria
AU - Xu, Ya Ming
AU - Ramirez-Ahumada, Maria del C.
AU - Valeriote, Frederick A.
AU - Gunatilaka, A. A.Leslie
N1 - Funding Information:
This work was supported by NIH research grant CA092143 awarded by the US National Cancer Institute. We thank Dr. David Newman of National Cancer Institute for providing the extract.
Funding Information:
[Received on] 03-Sep.-2011 [Research Funding] This project was supported by the US National Institutes of Health research grant CA092143 awarded by National Cancer Institute. [*Corresponding author] A.A.L. Gunatilaka: Prof., Tel: 520-621-9932, Fax: 520-621-8378, E-mail: [email protected]; F.A. Valeriote: Prof., E-mail: [email protected] These authors have no any conflict of interest to declare. Copyright © 2011, China Pharmaceutical University. Published by Elsevier B.V. All rights reserved.
PY - 2011/9
Y1 - 2011/9
N2 - Aim: To isolate and characterize solid tumor inhibitory and other constituents from a bioactive extract of Casimiroa tetrameria ((Rutaceae). Methods: A crude extract of C. tetrameria obtained from the US National Cancer Institute Natural Product Repository and found to exhibit selective toxicity to solid tumor cells was subjected bioactivity-guided fractionation involving solvent-solvent partitioning, gel filtration, and chromatography. The structures of all isolated compounds were elucidated by spectroscopic analysis (NMR and MS) and/or by comparison with the reported data. Compounds 1 and 4-9 were evaluated for their solid tumor selective cytotoxicity. Results: Nine metabolites, including a new furanocoumarin, 5-methoxy-8-(4'-acetoxy-3'-methylbut-2-enyloxy)-psoralen (1), and the previously known compounds 2-9 were encountered. Of these the flavonoid zapotin (6), and N-benzoyltyramide derivatives 7 and 8 were found to be the active constituents. Conclusion: Zapotin (6) is the most potent constituent of C. tetrameria with solid tumor selectivity.
AB - Aim: To isolate and characterize solid tumor inhibitory and other constituents from a bioactive extract of Casimiroa tetrameria ((Rutaceae). Methods: A crude extract of C. tetrameria obtained from the US National Cancer Institute Natural Product Repository and found to exhibit selective toxicity to solid tumor cells was subjected bioactivity-guided fractionation involving solvent-solvent partitioning, gel filtration, and chromatography. The structures of all isolated compounds were elucidated by spectroscopic analysis (NMR and MS) and/or by comparison with the reported data. Compounds 1 and 4-9 were evaluated for their solid tumor selective cytotoxicity. Results: Nine metabolites, including a new furanocoumarin, 5-methoxy-8-(4'-acetoxy-3'-methylbut-2-enyloxy)-psoralen (1), and the previously known compounds 2-9 were encountered. Of these the flavonoid zapotin (6), and N-benzoyltyramide derivatives 7 and 8 were found to be the active constituents. Conclusion: Zapotin (6) is the most potent constituent of C. tetrameria with solid tumor selectivity.
KW - Casimiroa tetrameria
KW - Flavonoids
KW - Furanocoumarins
KW - N-benzoyltyramides
KW - Rutaceae
KW - Solid tumor selectivity
KW - Zapotin
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U2 - 10.3724/SP.J.1009.2011.00334
DO - 10.3724/SP.J.1009.2011.00334
M3 - Article
AN - SCOPUS:80053250440
SN - 1875-5364
VL - 9
SP - 334
EP - 337
JO - Chinese Journal of Natural Medicines
JF - Chinese Journal of Natural Medicines
IS - 5
ER -