SOI technology-based microfiltration system for circulating tumor cells isolation and enumeration

Wei Ma, Ronghao Zeng, Dayu Liu, Yitshak Zohar, Yi Kuen Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Scopus citations

Abstract

A SOI technology-based micro filtration system for circulating tumor cells (CTCs) isolation and enumeration is reported; this is in contrast of using single-crystal-silicon wafers resulting in poor etching uniformity. This microsystem consists of a silicon filter enclosed with PDMS layers, a digital syringe pump, a fluorescence microscope, a digital CCD camera in combination with the CellProfiler software for rapid cell enumeration. Human cervical cancer cells (HeLa cells) were used for preliminary tests of the system performance. 3D CFD simulations demonstrate that the velocity and shear stress can be well controlled to avoid hydrodynamic cell lysis. In comparing with our previous CTC chip using polycarbonate (PC) filter membrane, the SOI-based system has a much improved CTC capturing and enumeration performance.

Original languageEnglish (US)
Title of host publication9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages333-336
Number of pages4
ISBN (Electronic)9781479947270
DOIs
StatePublished - Sep 23 2014
Event9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014 - Waikiki Beach, United States
Duration: Apr 13 2014Apr 16 2014

Publication series

Name9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014

Other

Other9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014
Country/TerritoryUnited States
CityWaikiki Beach
Period4/13/144/16/14

Keywords

  • SOI technology
  • circulating tumor cells (CTCs)
  • enrichment
  • isolation
  • microfiltration
  • microfluidic

ASJC Scopus subject areas

  • Control and Systems Engineering
  • Electrical and Electronic Engineering

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