Soevyǐ ingibitor Baumana-Birk kak affinnyǐ ligand dlia vydeleniia leǐkotsitarnoǐ élastazy. Ingibirovanie gidroliza élastina, kataliziruemogo leǐkotsitarnoǐ élastazoǐ.

T. V. Tikhonova; N. I. Larionova; I. P. Gladysheva; N. F. Kazanskaia

Soevyǐ ingibitor Baumana-Birk kak affinnyǐ ligand dlia vydeleniia leǐkotsitarnoǐ élastazy. Ingibirovanie gidroliza élastina, kataliziruemogo leǐkotsitarnoǐ élastazoǐ.

Translated title of the contribution: Bowman-Birk soy inhibitor as an affinity ligand for isolating leukocyte elastase. Inhibition of elastin hydrolysis, catalyzed by leukocyte elastase

T. V. Tikhonova, N. I. Larionova, I. P. Gladysheva, N. F. Kazanskaia

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

A one-step procedure for human leukocyte elastase purification using an affinity adsorbent based on protein soybean Bowman-Birk proteinase inhibitor has been developed. The leukocyte elastase was purified 70-fold with a 70-100% yield. The enzyme preparations did not contain cathepsin G and displayed a high specific activity. The soybean Bowman-Birk type inhibitor effectively inhibited the elastin hydrolysis by leukocyte elastase both when the enzyme and the inhibitor were simultaneously added to the substrate and after preliminary elastase adsorption on elastin. The inhibitory effect was preserved at high degrees of elastin hydrolysis.

Translated title of the contribution	Bowman-Birk soy inhibitor as an affinity ligand for isolating leukocyte elastase. Inhibition of elastin hydrolysis, catalyzed by leukocyte elastase
Original language	Russian
Pages (from-to)	1669-1676
Number of pages	8
Journal	Biokhimiya
Volume	58
Issue number	11
State	Published - Nov 1993
Externally published	Yes

ASJC Scopus subject areas

General Chemistry

Cite this

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title = "Soevyǐ ingibitor Baumana-Birk kak affinnyǐ ligand dlia vydeleniia leǐkotsitarnoǐ {\'e}lastazy. Ingibirovanie gidroliza {\'e}lastina, kataliziruemogo leǐkotsitarnoǐ {\'e}lastazoǐ.",

abstract = "A one-step procedure for human leukocyte elastase purification using an affinity adsorbent based on protein soybean Bowman-Birk proteinase inhibitor has been developed. The leukocyte elastase was purified 70-fold with a 70-100% yield. The enzyme preparations did not contain cathepsin G and displayed a high specific activity. The soybean Bowman-Birk type inhibitor effectively inhibited the elastin hydrolysis by leukocyte elastase both when the enzyme and the inhibitor were simultaneously added to the substrate and after preliminary elastase adsorption on elastin. The inhibitory effect was preserved at high degrees of elastin hydrolysis.",

author = "Tikhonova, {T. V.} and Larionova, {N. I.} and Gladysheva, {I. P.} and Kazanskaia, {N. F.}",

year = "1993",

month = nov,

language = "Russian",

volume = "58",

pages = "1669--1676",

journal = "Biokhimiya",

issn = "0320-9725",

publisher = "Izdatel'stvo Nauka",

number = "11",

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TY - JOUR

T1 - Soevyǐ ingibitor Baumana-Birk kak affinnyǐ ligand dlia vydeleniia leǐkotsitarnoǐ élastazy. Ingibirovanie gidroliza élastina, kataliziruemogo leǐkotsitarnoǐ élastazoǐ.

AU - Tikhonova, T. V.

AU - Larionova, N. I.

AU - Gladysheva, I. P.

AU - Kazanskaia, N. F.

PY - 1993/11

Y1 - 1993/11

N2 - A one-step procedure for human leukocyte elastase purification using an affinity adsorbent based on protein soybean Bowman-Birk proteinase inhibitor has been developed. The leukocyte elastase was purified 70-fold with a 70-100% yield. The enzyme preparations did not contain cathepsin G and displayed a high specific activity. The soybean Bowman-Birk type inhibitor effectively inhibited the elastin hydrolysis by leukocyte elastase both when the enzyme and the inhibitor were simultaneously added to the substrate and after preliminary elastase adsorption on elastin. The inhibitory effect was preserved at high degrees of elastin hydrolysis.

AB - A one-step procedure for human leukocyte elastase purification using an affinity adsorbent based on protein soybean Bowman-Birk proteinase inhibitor has been developed. The leukocyte elastase was purified 70-fold with a 70-100% yield. The enzyme preparations did not contain cathepsin G and displayed a high specific activity. The soybean Bowman-Birk type inhibitor effectively inhibited the elastin hydrolysis by leukocyte elastase both when the enzyme and the inhibitor were simultaneously added to the substrate and after preliminary elastase adsorption on elastin. The inhibitory effect was preserved at high degrees of elastin hydrolysis.

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ER -

Soevyǐ ingibitor Baumana-Birk kak affinnyǐ ligand dlia vydeleniia leǐkotsitarnoǐ élastazy. Ingibirovanie gidroliza élastina, kataliziruemogo leǐkotsitarnoǐ élastazoǐ.

Abstract

ASJC Scopus subject areas

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