TY - JOUR
T1 - Sodium selenite increases the activity of the tumor suppressor protein, PTEN, in DU-145 prostate cancer cells
AU - Berggren, Margareta
AU - Sittadjody, Sivanandane
AU - Song, Zuohe
AU - Samira, Jean Louis
AU - Burd, Randy
AU - Meuillet, Emmanuelle J.
PY - 2009/5
Y1 - 2009/5
N2 - Epidemiological and clinical data suggest that selenium may prevent prostate cancer; however, the cellular effects of selenium in malignant prostate cells are not well understood. We previously reported that the activity of the tumor suppressor PTEN is modulated by thioredoxin (Trx) in a RedOx-dependent manner. In this study, we demonstrated that the activity of Trx reductase (TR) is increased by sevenfold in the human prostate cancer cell line, DU-145, after 5 days of sodium selenite (Se) treatment. The treatment of DU-145 cells with increasing concentrations of Se induced an increase in PTEN lipid phosphatase activity by twofold, which correlated with a decrease in phospho-ser473-Akt, and an increase in phospho-Ser370-PTEN levels. Se also increased casein kinase-2 (CK2) activity; and the use of apigenin, an inhibitor of CK2, revealed that the regulation of the tumor suppressor PTEN by Se may be achieved via both the Trx-TR system and the RedOx control of the kinase involved in the regulation of PTEN activity.
AB - Epidemiological and clinical data suggest that selenium may prevent prostate cancer; however, the cellular effects of selenium in malignant prostate cells are not well understood. We previously reported that the activity of the tumor suppressor PTEN is modulated by thioredoxin (Trx) in a RedOx-dependent manner. In this study, we demonstrated that the activity of Trx reductase (TR) is increased by sevenfold in the human prostate cancer cell line, DU-145, after 5 days of sodium selenite (Se) treatment. The treatment of DU-145 cells with increasing concentrations of Se induced an increase in PTEN lipid phosphatase activity by twofold, which correlated with a decrease in phospho-ser473-Akt, and an increase in phospho-Ser370-PTEN levels. Se also increased casein kinase-2 (CK2) activity; and the use of apigenin, an inhibitor of CK2, revealed that the regulation of the tumor suppressor PTEN by Se may be achieved via both the Trx-TR system and the RedOx control of the kinase involved in the regulation of PTEN activity.
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U2 - 10.1080/01635580802521338
DO - 10.1080/01635580802521338
M3 - Article
C2 - 19373605
AN - SCOPUS:67649396127
VL - 61
SP - 322
EP - 331
JO - Nutrition and Cancer
JF - Nutrition and Cancer
SN - 0163-5581
IS - 3
ER -