Snapshot hyperspectral imaging in ophthalmology

William R. Johnson, Daniel W. Wilson, Wolfgang Fink, Mark Humayun, Greg Bearman

Research output: Contribution to journalArticlepeer-review

203 Scopus citations


Retinal imaging spectroscopy can provide functional maps using chromophore spectra. For example, oxygen saturation maps show ischemic areas from diabetes and venous occlusions. Obtaining retinal spatial-spectral data has been difficult due to saccades and long data acquisition times (>5 s). We present a snapshot imaging spectrometer with far-reaching applicability that acquires a complete spatial-spectral image cube in ∼3 ms from 450 to 700 nm with 50 bands, eliminating motion artifacts and pixel misregistration. Current retinal spectral imaging approaches are incapable of true snapshot operation over a wide spectral range with a large number of spectral bands. Coupled to a fundus camera, the instrument returns true color retinal images for comparison to standard fundus images and for image validation while the patient is still dilated. Oxygen saturation maps were obtained with a three-wavelength algorithm: for healthy subjects arteries were ∼95% and veins 30 to 35% less. The instrument is now undergoing clinical trials.

Original languageEnglish (US)
Article number014036-1
JournalJournal of biomedical optics
Issue number1
StatePublished - Jan 2007


  • Oximetry
  • Retina
  • Spectral imaging
  • Spectroscopy

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Biomedical Engineering


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