Smartphone epifluorescence microscopy for cellular imaging of fresh tissue in low-resource settings

Wenbin Zhu; Giacomo Pirovano; Patrick K. O’Neal; Cheng Gong; Nachiket Kulkarni; Christopher D. Nguyen; Christian Brand; Thomas Reiner; Dongkyun Kang

doi:10.1364/BOE.11.000089

Smartphone epifluorescence microscopy for cellular imaging of fresh tissue in low-resource settings

Wenbin Zhu, Giacomo Pirovano, Patrick K. O’Neal, Cheng Gong, Nachiket Kulkarni, Christopher D. Nguyen, Christian Brand, Thomas Reiner, Dongkyun Kang

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Disease diagnosis in low-resource settings can be challenging due to the lack of equipment and trained personnel required for histologic analysis. In this paper, we have developed a smartphone-based epifluorescence microscope (SeFM) for imaging fresh tissues at sub-cellular resolution. SeFM provides similar resolution and field of view (FOV) as those used during histologic analysis. The SeFM device achieved the lateral resolution of 0.57 µm and provided microscopy images over a sample area larger than 500 µm. The material cost was low, approximately $3,000. Preliminary images of human pancreatic tumor specimens clearly visualized cellular details. Quantitative analysis showed that using an excess dose of a chemotherapy drug significantly reduced the tumor-specific fluorescence signal, confirming the specificity of the drug and the detection potential of SeFM.

Original language	English (US)
Pages (from-to)	89-98
Number of pages	10
Journal	Biomedical Optics Express
Volume	11
Issue number	1
DOIs	https://doi.org/10.1364/BOE.11.000089
State	Published - 2020

ASJC Scopus subject areas

Biotechnology
Atomic and Molecular Physics, and Optics

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title = "Smartphone epifluorescence microscopy for cellular imaging of fresh tissue in low-resource settings",

abstract = "Disease diagnosis in low-resource settings can be challenging due to the lack of equipment and trained personnel required for histologic analysis. In this paper, we have developed a smartphone-based epifluorescence microscope (SeFM) for imaging fresh tissues at sub-cellular resolution. SeFM provides similar resolution and field of view (FOV) as those used during histologic analysis. The SeFM device achieved the lateral resolution of 0.57 µm and provided microscopy images over a sample area larger than 500 µm. The material cost was low, approximately $3,000. Preliminary images of human pancreatic tumor specimens clearly visualized cellular details. Quantitative analysis showed that using an excess dose of a chemotherapy drug significantly reduced the tumor-specific fluorescence signal, confirming the specificity of the drug and the detection potential of SeFM.",

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AU - Zhu, Wenbin

AU - Pirovano, Giacomo

AU - O’Neal, Patrick K.

AU - Gong, Cheng

AU - Kulkarni, Nachiket

AU - Nguyen, Christopher D.

AU - Brand, Christian

AU - Reiner, Thomas

AU - Kang, Dongkyun

PY - 2020

Y1 - 2020

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AB - Disease diagnosis in low-resource settings can be challenging due to the lack of equipment and trained personnel required for histologic analysis. In this paper, we have developed a smartphone-based epifluorescence microscope (SeFM) for imaging fresh tissues at sub-cellular resolution. SeFM provides similar resolution and field of view (FOV) as those used during histologic analysis. The SeFM device achieved the lateral resolution of 0.57 µm and provided microscopy images over a sample area larger than 500 µm. The material cost was low, approximately $3,000. Preliminary images of human pancreatic tumor specimens clearly visualized cellular details. Quantitative analysis showed that using an excess dose of a chemotherapy drug significantly reduced the tumor-specific fluorescence signal, confirming the specificity of the drug and the detection potential of SeFM.

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Smartphone epifluorescence microscopy for cellular imaging of fresh tissue in low-resource settings

Abstract

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