TY - JOUR
T1 - Smartphone epifluorescence microscopy for cellular imaging of fresh tissue in low-resource settings
AU - Zhu, Wenbin
AU - Pirovano, Giacomo
AU - O’Neal, Patrick K.
AU - Gong, Cheng
AU - Kulkarni, Nachiket
AU - Nguyen, Christopher D.
AU - Brand, Christian
AU - Reiner, Thomas
AU - Kang, Dongkyun
N1 - Funding Information:
National Cancer Institute (P30 CA008748, R01 CA204441, R43 CA228815). The authors thank the Tow Foundation and Memorial Sloan Kettering Cancer Center (MSKCC)?s Center for Molecular Imaging & Nanotechnology, Imaging and Radiation Sciences Program, and Molecularly Targeted Intraoperative Imaging Fund.
Publisher Copyright:
© 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.
PY - 2020
Y1 - 2020
N2 - Disease diagnosis in low-resource settings can be challenging due to the lack of equipment and trained personnel required for histologic analysis. In this paper, we have developed a smartphone-based epifluorescence microscope (SeFM) for imaging fresh tissues at sub-cellular resolution. SeFM provides similar resolution and field of view (FOV) as those used during histologic analysis. The SeFM device achieved the lateral resolution of 0.57 µm and provided microscopy images over a sample area larger than 500 µm. The material cost was low, approximately $3,000. Preliminary images of human pancreatic tumor specimens clearly visualized cellular details. Quantitative analysis showed that using an excess dose of a chemotherapy drug significantly reduced the tumor-specific fluorescence signal, confirming the specificity of the drug and the detection potential of SeFM.
AB - Disease diagnosis in low-resource settings can be challenging due to the lack of equipment and trained personnel required for histologic analysis. In this paper, we have developed a smartphone-based epifluorescence microscope (SeFM) for imaging fresh tissues at sub-cellular resolution. SeFM provides similar resolution and field of view (FOV) as those used during histologic analysis. The SeFM device achieved the lateral resolution of 0.57 µm and provided microscopy images over a sample area larger than 500 µm. The material cost was low, approximately $3,000. Preliminary images of human pancreatic tumor specimens clearly visualized cellular details. Quantitative analysis showed that using an excess dose of a chemotherapy drug significantly reduced the tumor-specific fluorescence signal, confirming the specificity of the drug and the detection potential of SeFM.
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U2 - 10.1364/BOE.11.000089
DO - 10.1364/BOE.11.000089
M3 - Article
AN - SCOPUS:85078886069
VL - 11
SP - 89
EP - 98
JO - Biomedical Optics Express
JF - Biomedical Optics Express
SN - 2156-7085
IS - 1
ER -