Abstract
SLP-76 is an adapter protein expressed in T cells and myeloid cells that is a substrate for ZAP-70 and Syk. SLP-76-deficient mice exhibit a profound block in T-cell development. We found that although SLP-76 is expressed in mouse mast cells, SLP-76(-/-) mice have normal numbers of mast cells in their skin and bronchi. SLP-76(-/-) mice are resistant to IgE-mediated passive anaphylaxis, SLP-76(-/-) mice sensitized with IgE anti-dinitrophenyl (DNP) and then challenged with DNP-HSA developed only mild and transient tachycardia, failed to increase their plasma histamine level, and all survived the antigen challenge. Bone marrow-derived mast cells (BMMCs) from SLP76(-/-) mice failed to release β-hexosaminidase and to secrete IL-6 after FcεRI cross-linking. Tyrosine phosphorylation of phospholipase C-γ1 (but not of Syk) and calcium mobilization in response to IgE cross-linking were reduced in SLP-76-deficient BMMCs. These results suggest that SLP-76 plays an important role in FcεRI-mediated signaling in mast cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1737-1743 |
| Number of pages | 7 |
| Journal | Journal of Clinical Investigation |
| Volume | 103 |
| Issue number | 12 |
| State | Published - Jun 1999 |
| Externally published | Yes |
ASJC Scopus subject areas
- General Medicine