Skin in the game: A review of single-cell and spatial transcriptomics in dermatological research

Samuel Schepps; Jonathan Xu; Henry Yang; Jenna Mandel; Jaanvi Mehta; Julianna Tolotta; Nicole Baker; Volkan Tekmen; Neda Nikbakht; Paolo Fortina; Ignacia Fuentes; Bonnie Lafleur; Raymond J. Cho; Andrew P. South

doi:10.1515/cclm-2023-1245

Skin in the game: A review of single-cell and spatial transcriptomics in dermatological research

Samuel Schepps, Jonathan Xu, Henry Yang, Jenna Mandel, Jaanvi Mehta, Julianna Tolotta, Nicole Baker, Volkan Tekmen, Neda Nikbakht, Paolo Fortina, Ignacia Fuentes, Bonnie Lafleur, Raymond J. Cho, Andrew P. South

Research output: Contribution to journal › Review article › peer-review

2 Scopus citations

Abstract

Single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics (ST) are two emerging research technologies that uniquely characterize gene expression microenvironments on a cellular or subcellular level. The skin, a clinically accessible tissue composed of diverse, essential cell populations, serves as an ideal target for these high-resolution investigative approaches. Using these tools, researchers are assembling a compendium of data and discoveries in healthy skin as well as a range of dermatologic pathophysiologies, including atopic dermatitis, psoriasis, and cutaneous malignancies. The ongoing advancement of single-cell approaches, coupled with anticipated decreases in cost with increased adoption, will reshape dermatologic research, profoundly influencing disease characterization, prognosis, and ultimately clinical practice.

Original language	English (US)
Pages (from-to)	1880-1891
Number of pages	12
Journal	Clinical Chemistry and Laboratory Medicine
Volume	62
Issue number	10
DOIs	https://doi.org/10.1515/cclm-2023-1245
State	Published - Sep 2024
Externally published	Yes

Keywords

gene expression
single cell transcriptomics
skin
spatial transcriptomics

ASJC Scopus subject areas

Clinical Biochemistry
Biochemistry, medical

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10.1515/cclm-2023-1245

Cite this

Schepps, S., Xu, J., Yang, H., Mandel, J., Mehta, J., Tolotta, J., Baker, N., Tekmen, V., Nikbakht, N., Fortina, P., Fuentes, I., Lafleur, B., Cho, R. J., & South, A. P. (2024). Skin in the game: A review of single-cell and spatial transcriptomics in dermatological research. Clinical Chemistry and Laboratory Medicine, 62(10), 1880-1891. https://doi.org/10.1515/cclm-2023-1245

Schepps, S, Xu, J, Yang, H, Mandel, J, Mehta, J, Tolotta, J, Baker, N, Tekmen, V, Nikbakht, N, Fortina, P, Fuentes, I, Lafleur, B, Cho, RJ & South, AP 2024, 'Skin in the game: A review of single-cell and spatial transcriptomics in dermatological research', Clinical Chemistry and Laboratory Medicine, vol. 62, no. 10, pp. 1880-1891. https://doi.org/10.1515/cclm-2023-1245

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AU - Baker, Nicole

AU - Tekmen, Volkan

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AB - Single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics (ST) are two emerging research technologies that uniquely characterize gene expression microenvironments on a cellular or subcellular level. The skin, a clinically accessible tissue composed of diverse, essential cell populations, serves as an ideal target for these high-resolution investigative approaches. Using these tools, researchers are assembling a compendium of data and discoveries in healthy skin as well as a range of dermatologic pathophysiologies, including atopic dermatitis, psoriasis, and cutaneous malignancies. The ongoing advancement of single-cell approaches, coupled with anticipated decreases in cost with increased adoption, will reshape dermatologic research, profoundly influencing disease characterization, prognosis, and ultimately clinical practice.

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Skin in the game: A review of single-cell and spatial transcriptomics in dermatological research

Abstract

Keywords

ASJC Scopus subject areas

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