Abstract
A protein nanoarray is created when submicro and nano beads, varying in their size and each conjugated with different proteins, self-assemble to specific locations depending on the diameter matching the surface electron beam patterns created. Protein binding is confirmed from the fluorescence attenuation of the beads upon antigen-antibody binding on the bead surface. This method, called size-dependent self-assembly, allows control of the location of each type of bead, and thus, control of the location of multiple proteins. It provides fast multi-component patterning with a high binding resolution, which can be detected using a fluorescent light microscope. This method is developed to be a simple stand-alone tool for analysis of protein interactions. In addition, it has the potential to be used in conjunction with other protein analysis methods, such as enzyme-linked immunosorbent assay (ELISA) and atomic force microscopy (AFM).
Original language | English (US) |
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Pages (from-to) | 2459-2463 |
Number of pages | 5 |
Journal | Materials Science and Engineering C |
Volume | 29 |
Issue number | 8 |
DOIs | |
State | Published - Oct 15 2009 |
Keywords
- Electron beam lithography
- Fluorescent attenuation
- Fluorescent beads
- Gold nanoparticles
- Protein nanoarray
- Self-assembly
ASJC Scopus subject areas
- General Medicine