Single step purification of platelet factor XIII using an immobilized factor XIII a-subunit monoclonal antibody

D. Lukacova; G. L. Reed

doi:10.1055/s-0038-1651620

Single step purification of platelet factor XIII using an immobilized factor XIII a-subunit monoclonal antibody

D. Lukacova, G. L. Reed

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

To facilitate the analysis of the catalytic subunit of factor XIII we have developed a method for the immunoaffinity purification of this protein from platelets. This method employs a monoclonal antibody that binds to the a-subunit of factor XIII. The anti-factor XIII antibody was immobilized on agarose and then incubated with platelet lysate. Subsequently factor XIII was isolated from the platelet lysate in a single step with a 41% yield as measured by enzyme assay. The purified platelet factor XIII appeared nearly homogeneous when analyzed by polyacrylamide electrophoresis and by immunoblotting with another factor XIII monoclonal antibody.

Original language	English (US)
Pages (from-to)	397-400
Number of pages	4
Journal	Thrombosis and Haemostasis
Volume	69
Issue number	4
DOIs	https://doi.org/10.1055/s-0038-1651620
State	Published - 1993
Externally published	Yes

ASJC Scopus subject areas

Hematology

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abstract = "To facilitate the analysis of the catalytic subunit of factor XIII we have developed a method for the immunoaffinity purification of this protein from platelets. This method employs a monoclonal antibody that binds to the a-subunit of factor XIII. The anti-factor XIII antibody was immobilized on agarose and then incubated with platelet lysate. Subsequently factor XIII was isolated from the platelet lysate in a single step with a 41% yield as measured by enzyme assay. The purified platelet factor XIII appeared nearly homogeneous when analyzed by polyacrylamide electrophoresis and by immunoblotting with another factor XIII monoclonal antibody.",

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year = "1993",

doi = "10.1055/s-0038-1651620",

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T1 - Single step purification of platelet factor XIII using an immobilized factor XIII a-subunit monoclonal antibody

AU - Lukacova, D.

AU - Reed, G. L.

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N2 - To facilitate the analysis of the catalytic subunit of factor XIII we have developed a method for the immunoaffinity purification of this protein from platelets. This method employs a monoclonal antibody that binds to the a-subunit of factor XIII. The anti-factor XIII antibody was immobilized on agarose and then incubated with platelet lysate. Subsequently factor XIII was isolated from the platelet lysate in a single step with a 41% yield as measured by enzyme assay. The purified platelet factor XIII appeared nearly homogeneous when analyzed by polyacrylamide electrophoresis and by immunoblotting with another factor XIII monoclonal antibody.

AB - To facilitate the analysis of the catalytic subunit of factor XIII we have developed a method for the immunoaffinity purification of this protein from platelets. This method employs a monoclonal antibody that binds to the a-subunit of factor XIII. The anti-factor XIII antibody was immobilized on agarose and then incubated with platelet lysate. Subsequently factor XIII was isolated from the platelet lysate in a single step with a 41% yield as measured by enzyme assay. The purified platelet factor XIII appeared nearly homogeneous when analyzed by polyacrylamide electrophoresis and by immunoblotting with another factor XIII monoclonal antibody.

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ER -

Single step purification of platelet factor XIII using an immobilized factor XIII a-subunit monoclonal antibody

Abstract

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