Simultaneous quantitative analysis of methyl salicylate, ethyl salicylate and salicylic acid from biological fluids using gas chromatography-mass spectrometry

Tarundeep Kakkar; Michael Mayersohn

doi:10.1016/S0378-4347(98)00348-X

Simultaneous quantitative analysis of methyl salicylate, ethyl salicylate and salicylic acid from biological fluids using gas chromatography-mass spectrometry

Tarundeep Kakkar, Michael Mayersohn

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

A gas chromatographic-mass spectrometric (GC-MS) assay was developed for the quantitative analysis of methyl salicylate (MeS), ethyl salicylate (ES) and salicylic acid (SA) from biological fluids. The method was validated from 100-μl rat liver homogenate preparations (5 mg/ml protein) in 70 mM KH₂PO₄ (pH 7.4) buffer and from 100 μl rat plasma. The samples were extracted with chloroform, derivatized with BSTFA and quantitated by GC-MS in the SIM mode. The standard curves ranged from 31 ng/ml to 800 or 1250 ng/ml. Relative standard deviations and bias were less than 11% in plasma and homogenate for all compounds except SA which evidenced greater variability. The assay was used in preliminary experiments to characterize the pharmacokinetics of MeS in rats. Copyright (C) 1998 Elsevier Science B.V.

Original language	English (US)
Pages (from-to)	69-75
Number of pages	7
Journal	Journal of Chromatography B: Biomedical Applications
Volume	718
Issue number	1
DOIs	https://doi.org/10.1016/S0378-4347(98)00348-X
State	Published - Oct 23 1998
Externally published	Yes

Keywords

Ethyl salicylate
Methyl salicylate
Salicylic acid

ASJC Scopus subject areas

General Chemistry

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10.1016/S0378-4347(98)00348-X

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title = "Simultaneous quantitative analysis of methyl salicylate, ethyl salicylate and salicylic acid from biological fluids using gas chromatography-mass spectrometry",

abstract = "A gas chromatographic-mass spectrometric (GC-MS) assay was developed for the quantitative analysis of methyl salicylate (MeS), ethyl salicylate (ES) and salicylic acid (SA) from biological fluids. The method was validated from 100-μl rat liver homogenate preparations (5 mg/ml protein) in 70 mM KH2PO4 (pH 7.4) buffer and from 100 μl rat plasma. The samples were extracted with chloroform, derivatized with BSTFA and quantitated by GC-MS in the SIM mode. The standard curves ranged from 31 ng/ml to 800 or 1250 ng/ml. Relative standard deviations and bias were less than 11% in plasma and homogenate for all compounds except SA which evidenced greater variability. The assay was used in preliminary experiments to characterize the pharmacokinetics of MeS in rats. Copyright (C) 1998 Elsevier Science B.V.",

keywords = "Ethyl salicylate, Methyl salicylate, Salicylic acid",

author = "Tarundeep Kakkar and Michael Mayersohn",

note = "Funding Information: The authors wish to thank Ms. Debra Martin for helpful suggestions in the development of the assay. The study was supported by a grant from the National Institute on Drugs of Abuse (DA 08094).",

year = "1998",

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doi = "10.1016/S0378-4347(98)00348-X",

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T1 - Simultaneous quantitative analysis of methyl salicylate, ethyl salicylate and salicylic acid from biological fluids using gas chromatography-mass spectrometry

AU - Kakkar, Tarundeep

AU - Mayersohn, Michael

N1 - Funding Information: The authors wish to thank Ms. Debra Martin for helpful suggestions in the development of the assay. The study was supported by a grant from the National Institute on Drugs of Abuse (DA 08094).

PY - 1998/10/23

Y1 - 1998/10/23

N2 - A gas chromatographic-mass spectrometric (GC-MS) assay was developed for the quantitative analysis of methyl salicylate (MeS), ethyl salicylate (ES) and salicylic acid (SA) from biological fluids. The method was validated from 100-μl rat liver homogenate preparations (5 mg/ml protein) in 70 mM KH2PO4 (pH 7.4) buffer and from 100 μl rat plasma. The samples were extracted with chloroform, derivatized with BSTFA and quantitated by GC-MS in the SIM mode. The standard curves ranged from 31 ng/ml to 800 or 1250 ng/ml. Relative standard deviations and bias were less than 11% in plasma and homogenate for all compounds except SA which evidenced greater variability. The assay was used in preliminary experiments to characterize the pharmacokinetics of MeS in rats. Copyright (C) 1998 Elsevier Science B.V.

AB - A gas chromatographic-mass spectrometric (GC-MS) assay was developed for the quantitative analysis of methyl salicylate (MeS), ethyl salicylate (ES) and salicylic acid (SA) from biological fluids. The method was validated from 100-μl rat liver homogenate preparations (5 mg/ml protein) in 70 mM KH2PO4 (pH 7.4) buffer and from 100 μl rat plasma. The samples were extracted with chloroform, derivatized with BSTFA and quantitated by GC-MS in the SIM mode. The standard curves ranged from 31 ng/ml to 800 or 1250 ng/ml. Relative standard deviations and bias were less than 11% in plasma and homogenate for all compounds except SA which evidenced greater variability. The assay was used in preliminary experiments to characterize the pharmacokinetics of MeS in rats. Copyright (C) 1998 Elsevier Science B.V.

KW - Ethyl salicylate

KW - Methyl salicylate

KW - Salicylic acid

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VL - 718

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EP - 75

JO - Journal of Chromatography B: Biomedical Applications

JF - Journal of Chromatography B: Biomedical Applications

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ER -

Simultaneous quantitative analysis of methyl salicylate, ethyl salicylate and salicylic acid from biological fluids using gas chromatography-mass spectrometry

Abstract

Keywords

ASJC Scopus subject areas

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