Simultaneous optical coherence tomography and laser induced fluorescence imaging in rat model of ovarian carcinogenesis

Lida P. Hariri, Erica R. Liebmann, Samuel L. Marion, Patricia B. Hoyer, John R. Davis, Molly A. Brewer, Jennifer K. Barton

Research output: Contribution to journalArticlepeer-review

37 Scopus citations


Determining if an ovarian mass is benign or malignant is an ongoing clinical challenge. The development of reliable animal models provides means to evaluate new diagnostic tools to more accurately determine if an ovary has benign or malignant features. Although sex cord-stromal tumors (SCS T) account for 0.1-0.5% of ovarian malignancies, they have similar appearances to more aggressive epithelial cancers and can serve as a prototype for developing better diagnostic methods for ovarian cancer. Optical coherence tomography (OCT) and laser-induced fluorescence (LIF) spectroscopy are non-destructive optical imaging modalities. OCT provides architectural cross-sectional images at near histological resolutions and LIF provides biochemical information. We utilize combined OCT-LIF to image ovaries in post-menopausal ovarian carcinogenesis rat models, evaluating normal cyclic, acyclic and neoplastic ovaries. Eighty-three female Fisher rats were exposed to combinations of control sesame oil, 4-vinylcyclohexene diepoxide (VCD) to induce ovarian failure, and/or 7,12-dimethylbenz[a]anthracene (DMBA) to induce carcinogenesis. Three or five months post-treatment, 162 ovaries were harvested and imaged with OCT-LIF: 40 cyclic, 105 acyclic and 17 SCS T. OCT identified various follicle stages, corpora lutea (CL), CL remnants, epithelial invaginations/inclusions and allowed for characterization of both cystic and solid SCS T. Signal attenuation comparisons between CL and solid SCS T revealed statistically significant increases in attenuation among CL. LIF characterized spectral differences in cyclic, acyclic and neoplastic ovaries attributed to collagen, NADH/FAD and hemoglobin absorption. We present combined OCT-LIF imaging in a rat ovarian carcinogenesis model, providing preliminary criteria for normal cyclic, acyclic and SCS T ovaries which support the potential of OCT-LIF for ovarian imaging.

Original languageEnglish (US)
Pages (from-to)438-447
Number of pages10
JournalCancer Biology and Therapy
Issue number5
StatePublished - Sep 1 2010


  • Fluorescence spectroscopy
  • Optical coherence tomography
  • Optical imaging
  • Ovarian cancer
  • Post-menopausal rat model

ASJC Scopus subject areas

  • Molecular Medicine
  • Oncology
  • Pharmacology
  • Cancer Research


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