TY - GEN
T1 - Simultaneous optical coherence and multiphoton microscopy of skin-equivalent tissue models
AU - Barton, Jennifer K.
AU - Tangb, Shuo
AU - Limb, Ryan
AU - Tromberg, Bruce J.
PY - 2007/1/1
Y1 - 2007/1/1
N2 - Three-layer skin-equivalent models (rafts) were created consisting of a collagen/fibroblast layer and an airexposed keratinocyte layer. Rafts were imaged with a tri-modality microscope including optical coherence (OC), two-photon excited fluorescence (TPEF), and second harmonic generation (SHG) channels. Some rafts were stained with Hoechst 33343 or rhodamine 123, and some were exposed to dimethyl sulfoxide (DMSO). OC microscopy revealed signal in cell cytoplasm and nuclear membranes, and a characteristic texture in the collagen/fibroblast layer. TPEF showed signal in cell cytoplasm and from collagen, and stained specimens revealed cell nuclei or mitochondria. There was little SHG in the keratinocyte layer, but strong signal from collagen bundles. Endogenous signals were severely attenuated in DMSO treated rafts; stained samples revealed shrunken and distorted cell structure. OC, TPEF, and SHG can provide complementary and nondestructive information about raft structure and effect of chemical agents.
AB - Three-layer skin-equivalent models (rafts) were created consisting of a collagen/fibroblast layer and an airexposed keratinocyte layer. Rafts were imaged with a tri-modality microscope including optical coherence (OC), two-photon excited fluorescence (TPEF), and second harmonic generation (SHG) channels. Some rafts were stained with Hoechst 33343 or rhodamine 123, and some were exposed to dimethyl sulfoxide (DMSO). OC microscopy revealed signal in cell cytoplasm and nuclear membranes, and a characteristic texture in the collagen/fibroblast layer. TPEF showed signal in cell cytoplasm and from collagen, and stained specimens revealed cell nuclei or mitochondria. There was little SHG in the keratinocyte layer, but strong signal from collagen bundles. Endogenous signals were severely attenuated in DMSO treated rafts; stained samples revealed shrunken and distorted cell structure. OC, TPEF, and SHG can provide complementary and nondestructive information about raft structure and effect of chemical agents.
KW - Dimethyl sulfoxide
KW - Fluorescence imaging
KW - Image contrast
KW - Multiphoton microscopy
KW - Optical coherence tomography
KW - Skin
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M3 - Conference contribution
SN - 9780819467713
T3 - Optics InfoBase Conference Papers
BT - European Conference on Biomedical Optics, ECBO 2007
PB - Optical Society of America
T2 - European Conference on Biomedical Optics, ECBO 2007
Y2 - 17 June 2007 through 17 June 2007
ER -