Simultaneous arsenic- and selenium-specific detection of the dimethyldiselenoarsinate anion by high-performance liquid chromatography-inductively coupled plasma atomic emission spectrometry

J. Gailer; G. A. Buttigieg; M. B. Denton

doi:10.1002/aoc.477

Simultaneous arsenic- and selenium-specific detection of the dimethyldiselenoarsinate anion by high-performance liquid chromatography-inductively coupled plasma atomic emission spectrometry

J. Gailer
, G. A. Buttigieg
, M. B. Denton

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

The seleno-bis (S-glutathionyl) arsinium ion, [(GS)₂AsSe]^-, which can be synthesized from arsenite, selenite and glutathione (GSH) at physiological pH, fundamentally links the mammalian metabolism of arsenite with that of selenite and is potentially involved in the chronic toxicity/carcinogenicity of inorganic arsenic. A mammalian metabolite of inorganic arsenic, dimethylarsinic acid, reacts with selenite and GSH in a similar manner to form the dimethyldiselenoarsinate anion, [(CH₃)₂As(Se)₂]^-. Since dimethylarsinic acid is an environmentally abundant arsenic compound that could interfere with the mammalian metabolism of the essential trace element selenium via the in vivo formation of [(CH₃)₂As(Se)₂]^-, a chromatographic method was developed to rapidly identify this compound in aqueous samples. Using an inductively coupled plasma atomic emission spectrometer (ICP-AES) as the simultaneous arsenic- and selenium-specific detector, the chromatographic retention behaviour of [(CH₃)₂As(Se)₂]^- was investigated on styrene-divinylbenzene-based high-performance liquid chromatography (HPLC) columns. With a Hamilton PRP-1 column as the stationary phase (250 × 4.1 mm ID, equipped with a guard column) and a phosphate-buffered saline buffer (0.01 mol dm^-3, pH 7.4) as the mobile phase, [(CH₃)₂As(Se)₂]^- was identified in the column effluent according to its arsenic: selenium molar ratio of 1:2. With this stationary phase/mobile phase combination, [(CH₃)₂As(Se)₂]^- was baseline-separated from arsenite, selenite, dimethylarsinate, methylarsonate and low molecular weight thiols (GSH, oxidized GSH) that are frequently encountered in biological samples. Thus, the HPLC-ICP-AES method developed should be useful for rapid identification and quantification of [(CH₃)₂As(Se)₂]^- in biological fluids.

Original language	English (US)
Pages (from-to)	570-574
Number of pages	5
Journal	Applied Organometallic Chemistry
Volume	17
Issue number	8
DOIs	https://doi.org/10.1002/aoc.477
State	Published - Aug 1 2003
Externally published	Yes

Keywords

Dimethyldiselenoarsinate anion
HPLC
ICP-AES

ASJC Scopus subject areas

General Chemistry
Inorganic Chemistry

Access to Document

10.1002/aoc.477

Cite this

Simultaneous arsenic- and selenium-specific detection of the dimethyldiselenoarsinate anion by high-performance liquid chromatography-inductively coupled plasma atomic emission spectrometry. / Gailer, J.; Buttigieg, G. A.; Denton, M. B.
In: Applied Organometallic Chemistry, Vol. 17, No. 8, 01.08.2003, p. 570-574.

Research output: Contribution to journal › Article › peer-review

@article{d4603627fe5b44368d9917f5be0fec20,

title = "Simultaneous arsenic- and selenium-specific detection of the dimethyldiselenoarsinate anion by high-performance liquid chromatography-inductively coupled plasma atomic emission spectrometry",

abstract = "The seleno-bis (S-glutathionyl) arsinium ion, [(GS)2AsSe]-, which can be synthesized from arsenite, selenite and glutathione (GSH) at physiological pH, fundamentally links the mammalian metabolism of arsenite with that of selenite and is potentially involved in the chronic toxicity/carcinogenicity of inorganic arsenic. A mammalian metabolite of inorganic arsenic, dimethylarsinic acid, reacts with selenite and GSH in a similar manner to form the dimethyldiselenoarsinate anion, [(CH3)2As(Se)2]-. Since dimethylarsinic acid is an environmentally abundant arsenic compound that could interfere with the mammalian metabolism of the essential trace element selenium via the in vivo formation of [(CH3)2As(Se)2]-, a chromatographic method was developed to rapidly identify this compound in aqueous samples. Using an inductively coupled plasma atomic emission spectrometer (ICP-AES) as the simultaneous arsenic- and selenium-specific detector, the chromatographic retention behaviour of [(CH3)2As(Se)2]- was investigated on styrene-divinylbenzene-based high-performance liquid chromatography (HPLC) columns. With a Hamilton PRP-1 column as the stationary phase (250 {\texttimes} 4.1 mm ID, equipped with a guard column) and a phosphate-buffered saline buffer (0.01 mol dm-3, pH 7.4) as the mobile phase, [(CH3)2As(Se)2]- was identified in the column effluent according to its arsenic: selenium molar ratio of 1:2. With this stationary phase/mobile phase combination, [(CH3)2As(Se)2]- was baseline-separated from arsenite, selenite, dimethylarsinate, methylarsonate and low molecular weight thiols (GSH, oxidized GSH) that are frequently encountered in biological samples. Thus, the HPLC-ICP-AES method developed should be useful for rapid identification and quantification of [(CH3)2As(Se)2]- in biological fluids.",

keywords = "Dimethyldiselenoarsinate anion, HPLC, ICP-AES",

author = "J. Gailer and Buttigieg, \{G. A.\} and Denton, \{M. B.\}",

year = "2003",

month = aug,

day = "1",

doi = "10.1002/aoc.477",

language = "English (US)",

volume = "17",

pages = "570--574",

journal = "Applied Organometallic Chemistry",

issn = "0268-2605",

publisher = "John Wiley and Sons Ltd",

number = "8",

}

TY - JOUR

T1 - Simultaneous arsenic- and selenium-specific detection of the dimethyldiselenoarsinate anion by high-performance liquid chromatography-inductively coupled plasma atomic emission spectrometry

AU - Gailer, J.

AU - Buttigieg, G. A.

AU - Denton, M. B.

PY - 2003/8/1

Y1 - 2003/8/1

N2 - The seleno-bis (S-glutathionyl) arsinium ion, [(GS)2AsSe]-, which can be synthesized from arsenite, selenite and glutathione (GSH) at physiological pH, fundamentally links the mammalian metabolism of arsenite with that of selenite and is potentially involved in the chronic toxicity/carcinogenicity of inorganic arsenic. A mammalian metabolite of inorganic arsenic, dimethylarsinic acid, reacts with selenite and GSH in a similar manner to form the dimethyldiselenoarsinate anion, [(CH3)2As(Se)2]-. Since dimethylarsinic acid is an environmentally abundant arsenic compound that could interfere with the mammalian metabolism of the essential trace element selenium via the in vivo formation of [(CH3)2As(Se)2]-, a chromatographic method was developed to rapidly identify this compound in aqueous samples. Using an inductively coupled plasma atomic emission spectrometer (ICP-AES) as the simultaneous arsenic- and selenium-specific detector, the chromatographic retention behaviour of [(CH3)2As(Se)2]- was investigated on styrene-divinylbenzene-based high-performance liquid chromatography (HPLC) columns. With a Hamilton PRP-1 column as the stationary phase (250 × 4.1 mm ID, equipped with a guard column) and a phosphate-buffered saline buffer (0.01 mol dm-3, pH 7.4) as the mobile phase, [(CH3)2As(Se)2]- was identified in the column effluent according to its arsenic: selenium molar ratio of 1:2. With this stationary phase/mobile phase combination, [(CH3)2As(Se)2]- was baseline-separated from arsenite, selenite, dimethylarsinate, methylarsonate and low molecular weight thiols (GSH, oxidized GSH) that are frequently encountered in biological samples. Thus, the HPLC-ICP-AES method developed should be useful for rapid identification and quantification of [(CH3)2As(Se)2]- in biological fluids.

AB - The seleno-bis (S-glutathionyl) arsinium ion, [(GS)2AsSe]-, which can be synthesized from arsenite, selenite and glutathione (GSH) at physiological pH, fundamentally links the mammalian metabolism of arsenite with that of selenite and is potentially involved in the chronic toxicity/carcinogenicity of inorganic arsenic. A mammalian metabolite of inorganic arsenic, dimethylarsinic acid, reacts with selenite and GSH in a similar manner to form the dimethyldiselenoarsinate anion, [(CH3)2As(Se)2]-. Since dimethylarsinic acid is an environmentally abundant arsenic compound that could interfere with the mammalian metabolism of the essential trace element selenium via the in vivo formation of [(CH3)2As(Se)2]-, a chromatographic method was developed to rapidly identify this compound in aqueous samples. Using an inductively coupled plasma atomic emission spectrometer (ICP-AES) as the simultaneous arsenic- and selenium-specific detector, the chromatographic retention behaviour of [(CH3)2As(Se)2]- was investigated on styrene-divinylbenzene-based high-performance liquid chromatography (HPLC) columns. With a Hamilton PRP-1 column as the stationary phase (250 × 4.1 mm ID, equipped with a guard column) and a phosphate-buffered saline buffer (0.01 mol dm-3, pH 7.4) as the mobile phase, [(CH3)2As(Se)2]- was identified in the column effluent according to its arsenic: selenium molar ratio of 1:2. With this stationary phase/mobile phase combination, [(CH3)2As(Se)2]- was baseline-separated from arsenite, selenite, dimethylarsinate, methylarsonate and low molecular weight thiols (GSH, oxidized GSH) that are frequently encountered in biological samples. Thus, the HPLC-ICP-AES method developed should be useful for rapid identification and quantification of [(CH3)2As(Se)2]- in biological fluids.

KW - Dimethyldiselenoarsinate anion

KW - HPLC

KW - ICP-AES

UR - https://www.scopus.com/pages/publications/0042265676

UR - https://www.scopus.com/pages/publications/0042265676#tab=citedBy

U2 - 10.1002/aoc.477

DO - 10.1002/aoc.477

M3 - Article

AN - SCOPUS:0042265676

SN - 0268-2605

VL - 17

SP - 570

EP - 574

JO - Applied Organometallic Chemistry

JF - Applied Organometallic Chemistry

IS - 8

ER -

Simultaneous arsenic- and selenium-specific detection of the dimethyldiselenoarsinate anion by high-performance liquid chromatography-inductively coupled plasma atomic emission spectrometry

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this