Simulation of stirred tank affinity processes applied to separation of proteins

Rosa Ma Montesinos, Roberto Guzmán, Armando Tejeda-Mansir

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


A model, which includes pore diffusion, external film resistance, and finite kinetic rate, was used to mathematically describe a batch affinity adsorption system. The corresponding differential equations system was solved using two numerical methods: the numerical method of lines (NUMOL) and the global (implicit) finite difference method. In each case, simulation studies were conducted to determine the mass-transfer-controlled mechanism. Experimental data from literature describing batch affinity adsorption of immunoglobulin G to protein A-Sepharose was used as a model system. The best fit of the experimental data was obtained with the mass-transfer process controlled by pore diffusion and film resistance, in the simulation studies, using the NUMOL solution. The transport model was used to perform a parametric analysis of the experimental batch system. The influence of both process parameters as well as physical parameters on the affinity adsorption process was investigated.

Original languageEnglish (US)
Pages (from-to)231-243
Number of pages13
JournalInternational Journal of Bio-Chromatography
Issue number3
StatePublished - 2001
Externally publishedYes


  • Adsorption
  • Batch affinity chromatography
  • Biomolecules
  • Mathematical modeling

ASJC Scopus subject areas

  • Biochemistry


Dive into the research topics of 'Simulation of stirred tank affinity processes applied to separation of proteins'. Together they form a unique fingerprint.

Cite this