Abstract
A model, which includes pore diffusion, external film resistance, and finite kinetic rate, was used to mathematically describe a batch affinity adsorption system. The corresponding differential equations system was solved using two numerical methods: the numerical method of lines (NUMOL) and the global (implicit) finite difference method. In each case, simulation studies were conducted to determine the mass-transfer-controlled mechanism. Experimental data from literature describing batch affinity adsorption of immunoglobulin G to protein A-Sepharose was used as a model system. The best fit of the experimental data was obtained with the mass-transfer process controlled by pore diffusion and film resistance, in the simulation studies, using the NUMOL solution. The transport model was used to perform a parametric analysis of the experimental batch system. The influence of both process parameters as well as physical parameters on the affinity adsorption process was investigated.
Original language | English (US) |
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Pages (from-to) | 231-243 |
Number of pages | 13 |
Journal | International Journal of Bio-Chromatography |
Volume | 6 |
Issue number | 3 |
State | Published - 2001 |
Externally published | Yes |
Keywords
- Adsorption
- Batch affinity chromatography
- Biomolecules
- Mathematical modeling
ASJC Scopus subject areas
- Biochemistry