Simplified protocol to isolate, purify, and culture expand mesenchymal stem cells

Sonja Schrepfer; Tobias Deuse; Claudia Lange; Regina Katzenberg; Hermann Reichenspurner; Robert C. Robbins; Marc P. Pelletier

doi:10.1089/scd.2006.0041

Simplified protocol to isolate, purify, and culture expand mesenchymal stem cells

Sonja Schrepfer
, Tobias Deuse
, Claudia Lange
, Regina Katzenberg
, Hermann Reichenspurner
, Robert C. Robbins
, Marc P. Pelletier

Research output: Contribution to journal › Article › peer-review

47 Scopus citations

Abstract

Mesenchymal stem cells (MSCs) are widely used for experimental regenerative strategies. Due to their differentiation capacity into mesenchymal lineages, they are a potential cellular source for tissue regeneration. Because there is no specific antigen that can be used to define MSCs directly, there is no consensus about how to isolate them. Here we describe a simple protocol to isolate, purify, and culture expand murine bone marrow MSCs using magnetic cell sorting and plastic adherence. We further show that cytokine supplementation enhances MSC proliferation without jeopardizing their pluripotency.

Original language	English (US)
Pages (from-to)	105-107
Number of pages	3
Journal	Stem Cells and Development
Volume	16
Issue number	1
DOIs	https://doi.org/10.1089/scd.2006.0041
State	Published - Feb 2007
Externally published	Yes

ASJC Scopus subject areas

Hematology
Developmental Biology
Cell Biology

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10.1089/scd.2006.0041

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AU - Deuse, Tobias

AU - Lange, Claudia

AU - Katzenberg, Regina

AU - Reichenspurner, Hermann

AU - Robbins, Robert C.

AU - Pelletier, Marc P.

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AB - Mesenchymal stem cells (MSCs) are widely used for experimental regenerative strategies. Due to their differentiation capacity into mesenchymal lineages, they are a potential cellular source for tissue regeneration. Because there is no specific antigen that can be used to define MSCs directly, there is no consensus about how to isolate them. Here we describe a simple protocol to isolate, purify, and culture expand murine bone marrow MSCs using magnetic cell sorting and plastic adherence. We further show that cytokine supplementation enhances MSC proliferation without jeopardizing their pluripotency.

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Simplified protocol to isolate, purify, and culture expand mesenchymal stem cells

Abstract

ASJC Scopus subject areas

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