TY - JOUR
T1 - Simplified protocol to isolate, purify, and culture expand mesenchymal stem cells
AU - Schrepfer, Sonja
AU - Deuse, Tobias
AU - Lange, Claudia
AU - Katzenberg, Regina
AU - Reichenspurner, Hermann
AU - Robbins, Robert C.
AU - Pelletier, Marc P.
PY - 2007/2
Y1 - 2007/2
N2 - Mesenchymal stem cells (MSCs) are widely used for experimental regenerative strategies. Due to their differentiation capacity into mesenchymal lineages, they are a potential cellular source for tissue regeneration. Because there is no specific antigen that can be used to define MSCs directly, there is no consensus about how to isolate them. Here we describe a simple protocol to isolate, purify, and culture expand murine bone marrow MSCs using magnetic cell sorting and plastic adherence. We further show that cytokine supplementation enhances MSC proliferation without jeopardizing their pluripotency.
AB - Mesenchymal stem cells (MSCs) are widely used for experimental regenerative strategies. Due to their differentiation capacity into mesenchymal lineages, they are a potential cellular source for tissue regeneration. Because there is no specific antigen that can be used to define MSCs directly, there is no consensus about how to isolate them. Here we describe a simple protocol to isolate, purify, and culture expand murine bone marrow MSCs using magnetic cell sorting and plastic adherence. We further show that cytokine supplementation enhances MSC proliferation without jeopardizing their pluripotency.
UR - http://www.scopus.com/inward/record.url?scp=33847623756&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33847623756&partnerID=8YFLogxK
U2 - 10.1089/scd.2006.0041
DO - 10.1089/scd.2006.0041
M3 - Article
C2 - 17348808
AN - SCOPUS:33847623756
SN - 1547-3287
VL - 16
SP - 105
EP - 107
JO - Stem Cells and Development
JF - Stem Cells and Development
IS - 1
ER -