Simplified protocol to isolate, purify, and culture expand mesenchymal stem cells

Sonja Schrepfer, Tobias Deuse, Claudia Lange, Regina Katzenberg, Hermann Reichenspurner, Robert C. Robbins, Marc P. Pelletier

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

Mesenchymal stem cells (MSCs) are widely used for experimental regenerative strategies. Due to their differentiation capacity into mesenchymal lineages, they are a potential cellular source for tissue regeneration. Because there is no specific antigen that can be used to define MSCs directly, there is no consensus about how to isolate them. Here we describe a simple protocol to isolate, purify, and culture expand murine bone marrow MSCs using magnetic cell sorting and plastic adherence. We further show that cytokine supplementation enhances MSC proliferation without jeopardizing their pluripotency.

Original languageEnglish (US)
Pages (from-to)105-107
Number of pages3
JournalStem Cells and Development
Volume16
Issue number1
DOIs
StatePublished - Feb 2007
Externally publishedYes

ASJC Scopus subject areas

  • Hematology
  • Developmental Biology
  • Cell Biology

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