Sigma factor-mediated plastid retrograde signals control nuclear gene expression

Jesse D. Woodson, Juan M. Perez-Ruiz, Robert J. Schmitz, Joseph R. Ecker, Joanne Chory

Research output: Contribution to journalArticlepeer-review

134 Scopus citations


Retrograde signalling from plastids to the nucleus is necessary to regulate the organelle's proteome during the establishment of photoautotrophy and fluctuating environmental conditions. Studies that used inhibitors of chloroplast biogenesis have revealed that hundreds of nuclear genes are regulated by retrograde signals emitted from plastids. Plastid gene expression is the source of at least one of these signals, but the number of signals and their mechanisms used to regulate nuclear gene expression are unknown. To further examine the effects of plastid gene expression on nuclear gene expression, we analyzed Arabidopsis mutants that were defective in each of the six sigma factor (SIG) genes that encode proteins utilized by plastid-encoded RNA polymerase to transcribe specific sets of plastid genes. We showed that SIG2 and SIG6 have partially redundant roles in plastid transcription and retrograde signalling to control nuclear gene expression. The loss of GUN1 (a plastid-localized pentatricopeptide repeat protein) is able to restore nuclear (but not plastid) gene expression in both sig2 and sig6, whereas an increase in heme synthesis is able to restore nuclear gene expression in sig2 mutants only. These results demonstrate that sigma factor function is the source of at least two retrograde signals to the nucleus; one likely to involve the transcription of tRNAGlu. A microarray analysis showed that these two signals accounted for at least one subset of the nuclear genes that are regulated by the plastid biogenesis inhibitors norflurazon and lincomycin. Together these data suggest that such inhibitors can induce retrograde signalling by affecting transcription in the plastid.

Original languageEnglish (US)
Pages (from-to)1-13
Number of pages13
JournalPlant Journal
Issue number1
StatePublished - Jan 2013
Externally publishedYes


  • Chloroplast
  • Photosynthesis
  • Retrograde signalling
  • Sigma factors
  • Tetrapyrroles
  • Transcription

ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Cell Biology


Dive into the research topics of 'Sigma factor-mediated plastid retrograde signals control nuclear gene expression'. Together they form a unique fingerprint.

Cite this