TY - JOUR
T1 - Shotgun optical maps of the whole Escherichia coli 0157:H7 genome
AU - Lim, Alex
AU - Dimalanta, Eileen T.
AU - Potamousis, Konstantinos D.
AU - Yen, Galex
AU - Apodoca, Jennifer
AU - Tao, Chunhong
AU - Lin, Jieyi
AU - Qi, Rong
AU - Skiadas, John
AU - Ramanathan, Arvind
AU - Perna, Nicole T.
AU - Plunkett, Guy
AU - Burland, Valerie
AU - Mau, Bob
AU - Hackett, Jeremiah
AU - Blattner, Frederick R.
AU - Anantharaman, Thomas S.
AU - Mishra, Bhubaneswar
AU - Schwartz, David C.
PY - 2001
Y1 - 2001
N2 - We have constructed Nhel and Xhol optical maps of Escherichia coli O157:H7 solely from genomic DNA molecules to provide a uniquely valuable scaffold for contig closure and sequence validation. E. coli O157:H7 is a common pathogen found in contaminated food and water. Our approach obviated the need for the analysis of clones, PCR products, and hybridizations, because maps were constructed from ensembles of single DNA molecules. Shotgun sequencing of bacterial genomes remains labor-intensive, despite advances in sequencing technology. This is partly due to manual intervention required during the last stages of finishing. The applicability of optical mapping to this problem was enhanced by advances in machine vision techniques that improved mapping throughput and created a path to full automation of mapping. Comparisons were made between maps and sequence data that characterized sequence gaps and guided nascent assemblies.
AB - We have constructed Nhel and Xhol optical maps of Escherichia coli O157:H7 solely from genomic DNA molecules to provide a uniquely valuable scaffold for contig closure and sequence validation. E. coli O157:H7 is a common pathogen found in contaminated food and water. Our approach obviated the need for the analysis of clones, PCR products, and hybridizations, because maps were constructed from ensembles of single DNA molecules. Shotgun sequencing of bacterial genomes remains labor-intensive, despite advances in sequencing technology. This is partly due to manual intervention required during the last stages of finishing. The applicability of optical mapping to this problem was enhanced by advances in machine vision techniques that improved mapping throughput and created a path to full automation of mapping. Comparisons were made between maps and sequence data that characterized sequence gaps and guided nascent assemblies.
UR - http://www.scopus.com/inward/record.url?scp=0034821826&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034821826&partnerID=8YFLogxK
U2 - 10.1101/gr.172101
DO - 10.1101/gr.172101
M3 - Article
C2 - 11544203
AN - SCOPUS:0034821826
VL - 11
SP - 1584
EP - 1593
JO - PCR Methods and Applications
JF - PCR Methods and Applications
SN - 1088-9051
IS - 9
ER -