@article{7ceb57eff76c41ab9a3dacd5b1bcfa20,
title = "Shed light in the dark lineages of the fungal tree of life—stres",
abstract = "The polyphyletic group of black fungi within the Ascomycota (Arthoniomycetes, Dothideomycetes, and Eurotiomycetes) is ubiquitous in natural and anthropogenic habitats. Partly because of their dark, melanin-based pigmentation, black fungi are resistant to stresses including UV-and ionizing-radiation, heat and desiccation, toxic metals, and organic pollutants. Consequently, they are amongst the most stunning extremophiles and poly-extreme-tolerant organisms on Earth. Even though ca. 60 black fungal genomes have been sequenced to date, [mostly in the family Herpotrichiellaceae (Eurotiomycetes)], the class Dothideomycetes that hosts the largest majority of extremophiles has only been sparsely sampled. By sequencing up to 92 species that will become reference genomes, the “Shed light in The daRk lineagES of the fungal tree of life” (STRES) project will cover a broad collection of black fungal diversity spread throughout the Fungal Tree of Life. Interestingly, the STRES project will focus on mostly unsampled genera that display different ecologies and life-styles (e.g., ant-and lichen-associated fungi, rock-inhabiting fungi, etc.). With a resequencing strategy of 10-to 15-fold depth coverage of up to ~550 strains, numerous new reference genomes will be established. To identify metabolites and functional processes, these new genomic resources will be enriched with metabolomics analyses coupled with transcriptomics experiments on selected species under various stress conditions (salinity, dryness, UV radiation, oligotrophy). The data acquired will serve as a reference and foundation for establishing an encyclopedic database for fungal metagenomics as well as the biology, evolution, and ecology of the fungi in extreme environments.",
keywords = "Adaptation, Black fungi, Dothideomycetes, Eurotiomycetes, Extremophiles, Genomics, Metabolomics, Secondary metabolites, Stress conditions, Transcriptomics",
author = "Laura Selbmann and Zsigmond Benk{\H o} and Claudia Coleine and {de Hoog}, Sybren and Claudio Donati and Irina Druzhinina and Tam{\'a}s Emri and Ettinger, {Cassie L.} and Gladfelter, {Amy S.} and Gorbushina, {Anna A.} and Grigoriev, {Igor V.} and Martin Grube and Nina Gunde-Cimerman and Kar{\'a}nyi, {Zsolt {\'A}kos} and Beatrix Kocsis and Tania Kubressoian and Ida Mikl{\'o}s and M{\'a}rton Miskei and Lucia Muggia and Trent Northen and Monika Novak-Babi{\v c} and Christa Pennacchio and Pfliegler, {Walter P.} and Istv{\`a}n P{\`o}csi and Valeria Prigione and Meritxell Riquelme and Nicola Segata and Julia Schumacher and Ekaterina Shelest and Katja Sterflinger and Donatella Tesei and U{\textquoteright}ren, {Jana M.} and Varese, {Giovanna C.} and Xabier V{\'a}zquez-Campos and Vicente, {Vania A.} and Souza, {Emanuel M.} and Polona Zalar and Walker, {Allison K.} and Stajich, {Jason E.}",
note = "Funding Information: Acknowledgments: Culture Collection of Antarctic fungi of the Mycological Section of the Italian Antarctic National Museum (MNA-FCC), Bundesanstalt f{\"u}r Materialforschung und -pr{\"u}fung collection (BAM), Culture Collection of Extremophilic Fungi (EXF), Austrian Center of Biological Resources and Applied Mycology (ACBR Collection), Microbiological Collections of Paran{\'a} Network (CMRP-Taxon line), Collection Mycotheca Universitatis Taurinensis (MUT) and all the personal Culture Collections are kindly acknowledged for financial support for preserving fungal strains involved in the STRES project. Funding Information: STRES (www.stresblackfungi.org) is a 3-year large-scale community science program project funded in September 2019 by the U.S. Department of Energy (DOE) Joint Genome Institute (JGI). funded in September 2019 by the U.S. Department of Energy (DOE) Joint Genome Institute (JGI). The STRES project will cover as best the amplitude of black fungal biodiversity along the The STRES project will cover as best the amplitude of black fungal biodiversity along the FTOL FTOL by sequencing up to 92 strains as reference genomes, representing primarily unsampled genera, by sequencing up to 92 strains as reference genomes, representing primarily unsampled genera, from from different ecologies and life-styles (e.g., ant-and lichen-associated fungi, rock-inhabiting fungi, etc.), different ecologies and life-styles (e.g., ant-and lichen-associated fungi, rock-inhabiting fungi, etc.), as well as more than 500 additional strains of black yeasts. We also proposed transcriptomics and as well as more than 500 additional strains of black yeasts. We also proposed transcriptomics and metabolomics experiments on a selection of reference species to track transcripts and expressed genes metabolomics experiments on a selection of reference species to track transcripts and expressed genes under different stress conditions (i.e., salinity, dryness, UV radiation, and oligotrophy) to further under different stress conditions (i.e., salinity, dryness, UV radiation, and oligotrophy) to further discern their roles in nutrient cycling, interactions in the environment, and to investigate the role of discern their roles in nutrient cycling, interactions in the environment, and to investigate the role of melanin in utilizing radiation as an energy source. The project workflow is outlined in Figure 3. melanin in utilizing radiation as an energy source. The project workflow is outlined in Figure 3. Funding Information: Funding: The work conducted by the U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231. J.E.S is a Fellow in the CIFAR program Fungal Kingdom: Threats and Opportunities. Publisher Copyright: {\textcopyright} 2020 by the authors. Licensee MDPI, Basel, Switzerland.",
year = "2020",
month = dec,
doi = "10.3390/life10120362",
language = "English (US)",
volume = "10",
pages = "1--13",
journal = "Life",
issn = "0024-3019",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "12",
}