TY - JOUR
T1 - Sex-based de novo transcriptome assemblies of the parasitoid wasp Encarsia suzannae, a host of the manipulative heritable symbiont Cardinium hertigii
AU - Schultz, Dylan L.
AU - Selberherr, Evelyne
AU - Stouthamer, Corinne M.
AU - Doremus, Matthew R.
AU - Kelly, Suzanne E.
AU - Hunter, Martha S.
AU - Schmitz-Esse, Stephan
N1 - Publisher Copyright:
© 2022 GigaScience Press. All Rights Reserved.
PY - 2022/9/2
Y1 - 2022/9/2
N2 - Parasitoid wasps in the genus Encarsia are commonly used as biological pest control agents of whiteflies and armored scale insects in greenhouses or the field. They are also hosts of the bacterial endosymbiont Cardinium hertigii, which can cause reproductive manipulation phenotypes, including parthenogenesis, feminization, and cytoplasmic incompatibility (the last is mainly studied in Encarsia suzannae). Despite their biological and economic importance, there are no published Encarsia genomes and only one public transcriptome. Here, we applied a mapping-and-removal approach to eliminate known contaminants from previously-obtained Illumina sequencing data. We generated de novo transcriptome assemblies for both female and male E. suzannae which contain 45,986 and 54,762 final coding sequences, respectively. Benchmarking Single-Copy Orthologs results indicate both assemblies are highly complete. Preliminary analyses revealed the presence of homologs of sex-determination genes characterized in other insects and putative venom proteins. Our male and female transcriptomes will be valuable tools to better understand the biology of Encarsia and their evolutionary relatives, particularly in studies involving insects of only one sex.
AB - Parasitoid wasps in the genus Encarsia are commonly used as biological pest control agents of whiteflies and armored scale insects in greenhouses or the field. They are also hosts of the bacterial endosymbiont Cardinium hertigii, which can cause reproductive manipulation phenotypes, including parthenogenesis, feminization, and cytoplasmic incompatibility (the last is mainly studied in Encarsia suzannae). Despite their biological and economic importance, there are no published Encarsia genomes and only one public transcriptome. Here, we applied a mapping-and-removal approach to eliminate known contaminants from previously-obtained Illumina sequencing data. We generated de novo transcriptome assemblies for both female and male E. suzannae which contain 45,986 and 54,762 final coding sequences, respectively. Benchmarking Single-Copy Orthologs results indicate both assemblies are highly complete. Preliminary analyses revealed the presence of homologs of sex-determination genes characterized in other insects and putative venom proteins. Our male and female transcriptomes will be valuable tools to better understand the biology of Encarsia and their evolutionary relatives, particularly in studies involving insects of only one sex.
KW - Animal Genetics
KW - Genetics and Genomics
KW - Transcriptomics
UR - http://www.scopus.com/inward/record.url?scp=85178198837&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85178198837&partnerID=8YFLogxK
U2 - 10.46471/gigabyte.68
DO - 10.46471/gigabyte.68
M3 - Article
AN - SCOPUS:85178198837
SN - 2709-4715
VL - 2022
JO - GigaByte
JF - GigaByte
ER -