Introduction. Envenomation by Centruroides sculpturatus can cause systemic signs and symptoms requiring treatment. The toxicokinetics of C. sculpturatus venom has not been described. Methods. Venom components were separated for cross-reactivity testing. Serum and urine collected from three patients envenomated by C. sculpturatus had venom levels determined by sandwich enzyme-linked immunosorbent assay (ELISA). Results. Western blot analysis indicated recognition of C. sculpturatus venom by Alacramyn®, an equine F(ab′)2 antivenom developed against Centruroides scorpion venoms, including C. sculpturatus. Serum venom levels in ng/mL with post-envenomation times in minutes (min) were as follows: 85-year-old woman = 8.2 (∼150), 2.8 (515), 1.6 (1,200); 14-month-old girl = 29.7 (∼50), 5.0 (729); 3-year-old girl = 11.1 (∼313), urine venom level of 9.0 (∼490). Conclusion. There is sufficient venom cross-antigenicity among different Centruroides species to allow this ELISA technique with Alacramyn® to determine serum and urine C. sculpturatus venom concentrations in envenomated patients.
- Centruroides sculpturatus
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