Abstract
Sedimentation field-flow fractionation has been used to separate myohemerythrin inclusion bodies from components of growth media, soluble proteins, and unlysed cells that are present in Escherichia coli cell lysates. Collected fractions were concentrated and then analyzed by sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis to confirm the presence of myohemerythrin inclusion bodies and to determine their position in the elution sequence. The fractograms of samples prepared using two different cell lysing methods were compared.
Original language | English (US) |
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Pages (from-to) | 233-239 |
Number of pages | 7 |
Journal | Journal of Microcolumn Separations |
Volume | 9 |
Issue number | 3 |
DOIs | |
State | Published - 1997 |
Keywords
- Cell lysates
- Field-flow fractionation
- Inclusion bodies
- Recombinant proteins
- Refractile bodies
ASJC Scopus subject areas
- Mechanical Engineering
- Filtration and Separation