Separation of Protein Inclusion Bodies from Escherichia coli Lysates Using Sedimentation Field-Flow Fractionation

S. Kim Ratanathanawongs Williams; Gregory M. Raner; Walther R. Ellis; J. Calvin Giddings

doi:10.1002/(SICI)1520-667X(1997)9:3<233::AID-MCS12>3.0.CO;2-9

Separation of Protein Inclusion Bodies from Escherichia coli Lysates Using Sedimentation Field-Flow Fractionation

S. Kim Ratanathanawongs Williams, Gregory M. Raner, Walther R. Ellis, J. Calvin Giddings

Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Sedimentation field-flow fractionation has been used to separate myohemerythrin inclusion bodies from components of growth media, soluble proteins, and unlysed cells that are present in Escherichia coli cell lysates. Collected fractions were concentrated and then analyzed by sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis to confirm the presence of myohemerythrin inclusion bodies and to determine their position in the elution sequence. The fractograms of samples prepared using two different cell lysing methods were compared.

Original language	English (US)
Pages (from-to)	233-239
Number of pages	7
Journal	Journal of Microcolumn Separations
Volume	9
Issue number	3
DOIs	https://doi.org/10.1002/(SICI)1520-667X(1997)9:3<233::AID-MCS12>3.0.CO;2-9
State	Published - 1997

Keywords

Cell lysates
Field-flow fractionation
Inclusion bodies
Recombinant proteins
Refractile bodies

ASJC Scopus subject areas

Mechanical Engineering
Filtration and Separation

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10.1002/(SICI)1520-667X(1997)9:3<233::AID-MCS12>3.0.CO;2-9

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title = "Separation of Protein Inclusion Bodies from Escherichia coli Lysates Using Sedimentation Field-Flow Fractionation",

abstract = "Sedimentation field-flow fractionation has been used to separate myohemerythrin inclusion bodies from components of growth media, soluble proteins, and unlysed cells that are present in Escherichia coli cell lysates. Collected fractions were concentrated and then analyzed by sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis to confirm the presence of myohemerythrin inclusion bodies and to determine their position in the elution sequence. The fractograms of samples prepared using two different cell lysing methods were compared.",

keywords = "Cell lysates, Field-flow fractionation, Inclusion bodies, Recombinant proteins, Refractile bodies",

author = "{Ratanathanawongs Williams}, {S. Kim} and Raner, {Gregory M.} and Ellis, {Walther R.} and Giddings, {J. Calvin}",

year = "1997",

doi = "10.1002/(SICI)1520-667X(1997)9:3<233::AID-MCS12>3.0.CO;2-9",

language = "English (US)",

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pages = "233--239",

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T1 - Separation of Protein Inclusion Bodies from Escherichia coli Lysates Using Sedimentation Field-Flow Fractionation

AU - Ratanathanawongs Williams, S. Kim

AU - Raner, Gregory M.

AU - Ellis, Walther R.

AU - Giddings, J. Calvin

PY - 1997

Y1 - 1997

N2 - Sedimentation field-flow fractionation has been used to separate myohemerythrin inclusion bodies from components of growth media, soluble proteins, and unlysed cells that are present in Escherichia coli cell lysates. Collected fractions were concentrated and then analyzed by sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis to confirm the presence of myohemerythrin inclusion bodies and to determine their position in the elution sequence. The fractograms of samples prepared using two different cell lysing methods were compared.

AB - Sedimentation field-flow fractionation has been used to separate myohemerythrin inclusion bodies from components of growth media, soluble proteins, and unlysed cells that are present in Escherichia coli cell lysates. Collected fractions were concentrated and then analyzed by sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis to confirm the presence of myohemerythrin inclusion bodies and to determine their position in the elution sequence. The fractograms of samples prepared using two different cell lysing methods were compared.

KW - Cell lysates

KW - Field-flow fractionation

KW - Inclusion bodies

KW - Recombinant proteins

KW - Refractile bodies

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DO - 10.1002/(SICI)1520-667X(1997)9:3<233::AID-MCS12>3.0.CO;2-9

M3 - Article

AN - SCOPUS:0347802911

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ER -

Separation of Protein Inclusion Bodies from Escherichia coli Lysates Using Sedimentation Field-Flow Fractionation

Abstract

Keywords

ASJC Scopus subject areas

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