Sensitivity of nested PCR in the detection of low numbers of Giardia lamblia cysts

Kathryn M. Miller, Charles R. Sterling

    Research output: Contribution to journalArticlepeer-review

    23 Scopus citations

    Abstract

    Nested PCR was performed on individually isolated Giardia lamblia cysts in replicates of 50 for sets of 1, 2, 3, 4, 5, 7, and 10 cysts. Amplification ranged from 80% for 1 cyst to 100% for 10 cysts. The results suggest that nested PCR is well adapted for G. lamblia single-cyst detection.

    Original languageEnglish (US)
    Pages (from-to)5949-5950
    Number of pages2
    JournalApplied and environmental microbiology
    Volume73
    Issue number18
    DOIs
    StatePublished - Sep 2007

    ASJC Scopus subject areas

    • Biotechnology
    • Food Science
    • Applied Microbiology and Biotechnology
    • Ecology

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