Nested PCR was performed on individually isolated Giardia lamblia cysts in replicates of 50 for sets of 1, 2, 3, 4, 5, 7, and 10 cysts. Amplification ranged from 80% for 1 cyst to 100% for 10 cysts. The results suggest that nested PCR is well adapted for G. lamblia single-cyst detection.
ASJC Scopus subject areas
- Food Science
- Applied Microbiology and Biotechnology