Sensitivity of nested PCR in the detection of low numbers of Giardia lamblia cysts

Kathryn M. Miller; Charles R. Sterling

doi:10.1128/AEM.00668-07

Sensitivity of nested PCR in the detection of low numbers of Giardia lamblia cysts

Kathryn M. Miller, Charles R. Sterling

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Nested PCR was performed on individually isolated Giardia lamblia cysts in replicates of 50 for sets of 1, 2, 3, 4, 5, 7, and 10 cysts. Amplification ranged from 80% for 1 cyst to 100% for 10 cysts. The results suggest that nested PCR is well adapted for G. lamblia single-cyst detection.

Original language	English (US)
Pages (from-to)	5949-5950
Number of pages	2
Journal	Applied and environmental microbiology
Volume	73
Issue number	18
DOIs	https://doi.org/10.1128/AEM.00668-07
State	Published - Sep 2007

ASJC Scopus subject areas

Biotechnology
Food Science
Applied Microbiology and Biotechnology
Ecology

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title = "Sensitivity of nested PCR in the detection of low numbers of Giardia lamblia cysts",

abstract = "Nested PCR was performed on individually isolated Giardia lamblia cysts in replicates of 50 for sets of 1, 2, 3, 4, 5, 7, and 10 cysts. Amplification ranged from 80% for 1 cyst to 100% for 10 cysts. The results suggest that nested PCR is well adapted for G. lamblia single-cyst detection.",

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Sensitivity of nested PCR in the detection of low numbers of Giardia lamblia cysts

Abstract

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