Sensitivity- and Gradient-Enhanced Heteronuclear Coupled/Decoupled HSQC-TOCSY Experiments for Measuring Long-Range Heteronuclear Coupling Constants

Katalin E. Kövér; Victor J. Hruby; Dušan Uhrín

doi:10.1006/jmre.1997.1265

Sensitivity- and Gradient-Enhanced Heteronuclear Coupled/Decoupled HSQC-TOCSY Experiments for Measuring Long-Range Heteronuclear Coupling Constants

Katalin E. Kövér, Victor J. Hruby, Dušan Uhrín

Research output: Contribution to journal › Article › peer-review

66 Scopus citations

Abstract

A pulsed field gradient version of the sensitivity-enhanced 2D HSQC-TOCSY experiment is proposed for measurement of long-range heteronuclear coupling constants. The coupling constants are obtained by computer-aided analysis of mixed-phase multiplets with and without the heteronuclear splitting. Generation of pure phase data is not required. Since large ¹J_XH and J_HH couplings are used for coherence transfer, small ⁿJ_XH can be measured accurately, which could be difficult to obtain from purely heteronuclear polarization transfer experiments.

Original language	English (US)
Pages (from-to)	125-129
Number of pages	5
Journal	Journal of Magnetic Resonance
Volume	129
Issue number	2
DOIs	https://doi.org/10.1006/jmre.1997.1265
State	Published - Dec 1997
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Nuclear and High Energy Physics
Condensed Matter Physics

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10.1006/jmre.1997.1265

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title = "Sensitivity- and Gradient-Enhanced Heteronuclear Coupled/Decoupled HSQC-TOCSY Experiments for Measuring Long-Range Heteronuclear Coupling Constants",

abstract = "A pulsed field gradient version of the sensitivity-enhanced 2D HSQC-TOCSY experiment is proposed for measurement of long-range heteronuclear coupling constants. The coupling constants are obtained by computer-aided analysis of mixed-phase multiplets with and without the heteronuclear splitting. Generation of pure phase data is not required. Since large 1JXH and JHH couplings are used for coherence transfer, small nJXH can be measured accurately, which could be difficult to obtain from purely heteronuclear polarization transfer experiments.",

author = "K{\"o}v{\'e}r, {Katalin E.} and Hruby, {Victor J.} and Du{\v s}an Uhr{\'i}n",

note = "Funding Information: K.E.K. thanks the National Research Foundation for financial support (OTKA T 014982 and OTKA D 23749). The purchase of the spectrometer used in the study was supported by OMFB Mec-93-0098, Phare-Accord H-9112-0198, and OTKA A084. This work was, in part (D.U.), supported by the Wellcome Trust. The support from NIDA Grant DA 06284 and USPHS Grant DKI7420 is also acknowledged by V.J.H. We thank Dr. J. Hirsch for sample of the trisaccharide and Dr. E. J. Beatty for reading the manuscript.",

year = "1997",

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doi = "10.1006/jmre.1997.1265",

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AU - Kövér, Katalin E.

AU - Hruby, Victor J.

AU - Uhrín, Dušan

N1 - Funding Information: K.E.K. thanks the National Research Foundation for financial support (OTKA T 014982 and OTKA D 23749). The purchase of the spectrometer used in the study was supported by OMFB Mec-93-0098, Phare-Accord H-9112-0198, and OTKA A084. This work was, in part (D.U.), supported by the Wellcome Trust. The support from NIDA Grant DA 06284 and USPHS Grant DKI7420 is also acknowledged by V.J.H. We thank Dr. J. Hirsch for sample of the trisaccharide and Dr. E. J. Beatty for reading the manuscript.

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N2 - A pulsed field gradient version of the sensitivity-enhanced 2D HSQC-TOCSY experiment is proposed for measurement of long-range heteronuclear coupling constants. The coupling constants are obtained by computer-aided analysis of mixed-phase multiplets with and without the heteronuclear splitting. Generation of pure phase data is not required. Since large 1JXH and JHH couplings are used for coherence transfer, small nJXH can be measured accurately, which could be difficult to obtain from purely heteronuclear polarization transfer experiments.

AB - A pulsed field gradient version of the sensitivity-enhanced 2D HSQC-TOCSY experiment is proposed for measurement of long-range heteronuclear coupling constants. The coupling constants are obtained by computer-aided analysis of mixed-phase multiplets with and without the heteronuclear splitting. Generation of pure phase data is not required. Since large 1JXH and JHH couplings are used for coherence transfer, small nJXH can be measured accurately, which could be difficult to obtain from purely heteronuclear polarization transfer experiments.

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Sensitivity- and Gradient-Enhanced Heteronuclear Coupled/Decoupled HSQC-TOCSY Experiments for Measuring Long-Range Heteronuclear Coupling Constants

Abstract

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