Sensitive, smartphone-based SARS-CoV-2 detection from clinical saline gargle samples

Lane E. Breshears; Brandon T. Nguyen; Patarajarin Akarapipad; Katelyn Sosnowski; Kattika Kaarj; Grace Quirk; Jennifer L. Uhrlaub; Janko Nikolich-Žugich; Michael Worobey; Jeong Yeol Yoon

doi:10.1093/pnasnexus/pgac028

Sensitive, smartphone-based SARS-CoV-2 detection from clinical saline gargle samples

Lane E. Breshears, Brandon T. Nguyen, Patarajarin Akarapipad, Katelyn Sosnowski, Kattika Kaarj, Grace Quirk, Jennifer L. Uhrlaub, Janko Nikolich-Žugich, Michael Worobey, Jeong Yeol Yoon

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Saliva specimens have drawn interest for diagnosing respiratory viral infections due to their ease of collection and decreased risk to healthcare providers. However, rapid and sensitive immunoassays have not yet been satisfactorily demonstrated for such specimens due to their viscosity and low viral loads. Using paper microfluidic chips and a smartphone-based fluorescence microscope, we developed a highly sensitive, low-cost immunofluorescence particulometric SARS-CoV-2 assay from clinical saline gargle samples. We demonstrated the limit of detection of 10 ag/μL. With easy-to-collect saline gargle samples, our clinical sensitivity, specificity, and accuracy were 100%, 86%, and 93%, respectively, for n = 27 human subjects with n = 13 RT-qPCR positives.

Original language	English (US)
Article number	pgac028
Journal	PNAS Nexus
Volume	1
Issue number	1
DOIs	https://doi.org/10.1093/pnasnexus/pgac028
State	Published - Mar 1 2022

Keywords

COVID-19
biosensor
clinical samples
immunoassay
microfluidics
point-of-care
smartphone
smartphone-based fluorescence microscope

ASJC Scopus subject areas

General

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10.1093/pnasnexus/pgac028

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title = "Sensitive, smartphone-based SARS-CoV-2 detection from clinical saline gargle samples",

abstract = "Saliva specimens have drawn interest for diagnosing respiratory viral infections due to their ease of collection and decreased risk to healthcare providers. However, rapid and sensitive immunoassays have not yet been satisfactorily demonstrated for such specimens due to their viscosity and low viral loads. Using paper microfluidic chips and a smartphone-based fluorescence microscope, we developed a highly sensitive, low-cost immunofluorescence particulometric SARS-CoV-2 assay from clinical saline gargle samples. We demonstrated the limit of detection of 10 ag/μL. With easy-to-collect saline gargle samples, our clinical sensitivity, specificity, and accuracy were 100%, 86%, and 93%, respectively, for n = 27 human subjects with n = 13 RT-qPCR positives.",

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doi = "10.1093/pnasnexus/pgac028",

language = "English (US)",

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AU - Breshears, Lane E.

AU - Nguyen, Brandon T.

AU - Akarapipad, Patarajarin

AU - Sosnowski, Katelyn

AU - Kaarj, Kattika

AU - Quirk, Grace

AU - Uhrlaub, Jennifer L.

AU - Nikolich-Žugich, Janko

AU - Worobey, Michael

AU - Yoon, Jeong Yeol

N1 - Publisher Copyright: ©C The Author(s) 2022.

PY - 2022/3/1

Y1 - 2022/3/1

N2 - Saliva specimens have drawn interest for diagnosing respiratory viral infections due to their ease of collection and decreased risk to healthcare providers. However, rapid and sensitive immunoassays have not yet been satisfactorily demonstrated for such specimens due to their viscosity and low viral loads. Using paper microfluidic chips and a smartphone-based fluorescence microscope, we developed a highly sensitive, low-cost immunofluorescence particulometric SARS-CoV-2 assay from clinical saline gargle samples. We demonstrated the limit of detection of 10 ag/μL. With easy-to-collect saline gargle samples, our clinical sensitivity, specificity, and accuracy were 100%, 86%, and 93%, respectively, for n = 27 human subjects with n = 13 RT-qPCR positives.

AB - Saliva specimens have drawn interest for diagnosing respiratory viral infections due to their ease of collection and decreased risk to healthcare providers. However, rapid and sensitive immunoassays have not yet been satisfactorily demonstrated for such specimens due to their viscosity and low viral loads. Using paper microfluidic chips and a smartphone-based fluorescence microscope, we developed a highly sensitive, low-cost immunofluorescence particulometric SARS-CoV-2 assay from clinical saline gargle samples. We demonstrated the limit of detection of 10 ag/μL. With easy-to-collect saline gargle samples, our clinical sensitivity, specificity, and accuracy were 100%, 86%, and 93%, respectively, for n = 27 human subjects with n = 13 RT-qPCR positives.

KW - COVID-19

KW - biosensor

KW - clinical samples

KW - immunoassay

KW - microfluidics

KW - point-of-care

KW - smartphone

KW - smartphone-based fluorescence microscope

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Sensitive, smartphone-based SARS-CoV-2 detection from clinical saline gargle samples

Abstract

Keywords

ASJC Scopus subject areas

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