TY - JOUR
T1 - Sensitive and rapid isocratic liquid chromatography method for the quantitation of curcumin in plasma
AU - Pak, Yvonne
AU - Patek, Renata
AU - Mayersohn, Michael
N1 - Funding Information:
This study was supported by the Arizona Center for Phytomedicine Research (grant #1P50AT00474) from the National Institute of Health.
PY - 2003/11/5
Y1 - 2003/11/5
N2 - An HPLC assay was developed using three methods of plasma sample preparation in order to quantitate curcumin, the main constituent in the herbal dietary supplement turmeric. Each method involves simple and rapid processing of samples (either an ethyl acetate or chloroform extraction) with resulting different quantitation limits for curcumin. The assay was developed in an effort to quantify extremely low curcumin plasma concentrations observed in preliminary in vivo studies. The most sensitive assay can reliably detect concentrations down to 2.5ng/ml. Plasma quantitation was precise and accurate based on both intra- and inter-day validations as indicated by low values for coefficients of variation and bias, respectively (≤15%). The analytical validation was reproducible between different analysts. The resulting analytical method couples desired sensitivity with the ease of an isocratic system.
AB - An HPLC assay was developed using three methods of plasma sample preparation in order to quantitate curcumin, the main constituent in the herbal dietary supplement turmeric. Each method involves simple and rapid processing of samples (either an ethyl acetate or chloroform extraction) with resulting different quantitation limits for curcumin. The assay was developed in an effort to quantify extremely low curcumin plasma concentrations observed in preliminary in vivo studies. The most sensitive assay can reliably detect concentrations down to 2.5ng/ml. Plasma quantitation was precise and accurate based on both intra- and inter-day validations as indicated by low values for coefficients of variation and bias, respectively (≤15%). The analytical validation was reproducible between different analysts. The resulting analytical method couples desired sensitivity with the ease of an isocratic system.
KW - Curcumin
UR - http://www.scopus.com/inward/record.url?scp=0142258143&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0142258143&partnerID=8YFLogxK
U2 - 10.1016/j.jchromb.2003.08.018
DO - 10.1016/j.jchromb.2003.08.018
M3 - Article
C2 - 14581073
AN - SCOPUS:0142258143
SN - 1570-0232
VL - 796
SP - 339
EP - 346
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
IS - 2
ER -