TY - JOUR
T1 - Sensing small molecules by nascent RNA
T2 - A mechanism to control transcription in bacteria
AU - Mironov, Alexander S.
AU - Gusarov, Ivan
AU - Rafikov, Ruslan
AU - Lopez, Lubov Errais
AU - Shatalin, Konstantin
AU - Kreneva, Rimma A.
AU - Perumov, Daniel A.
AU - Nudler, Evgeny
N1 - Funding Information:
We thank R. Burgess for the B. subtilis His6-β′ fusion strain. This work was supported in part by the Russian Fund of Fundamental Investigation grant 00-O4-48868 (D.A.P) and Irma T. Hirschl Career Scientist Award, Edward Mallinckrodt, Jr. Foundation, and NIH grant GM58750 (E.N.).
PY - 2002/11/27
Y1 - 2002/11/27
N2 - Thiamin and riboflavin are precursors of essential coenzymes - thiamin pyrophosphate (TPP) and flavin mononucleotide (FMN)/flavin adenine dinucleotide (FAD), respectively. In Bacillus spp, genes responsible for thiamin and riboflavin biosynthesis are organized in tightly controllable operons. Here, we demonstrate that the feedback regulation of riboflavin and thiamin genes relies on a novel transcription attenuation mechanism. A unique feature of this mechanism is the formation of specific complexes between a conserved leader region of the cognate RNA and FMN or TPP. In each case, the complex allows the termination hairpin to form and interrupt transcription prematurely. Thus, sensing small molecules by nascent RNA controls transcription elongation of riboflavin and thiamin operons and possibly other bacterial operons as well.
AB - Thiamin and riboflavin are precursors of essential coenzymes - thiamin pyrophosphate (TPP) and flavin mononucleotide (FMN)/flavin adenine dinucleotide (FAD), respectively. In Bacillus spp, genes responsible for thiamin and riboflavin biosynthesis are organized in tightly controllable operons. Here, we demonstrate that the feedback regulation of riboflavin and thiamin genes relies on a novel transcription attenuation mechanism. A unique feature of this mechanism is the formation of specific complexes between a conserved leader region of the cognate RNA and FMN or TPP. In each case, the complex allows the termination hairpin to form and interrupt transcription prematurely. Thus, sensing small molecules by nascent RNA controls transcription elongation of riboflavin and thiamin operons and possibly other bacterial operons as well.
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U2 - 10.1016/S0092-8674(02)01134-0
DO - 10.1016/S0092-8674(02)01134-0
M3 - Article
C2 - 12464185
AN - SCOPUS:18744396047
SN - 0092-8674
VL - 111
SP - 747
EP - 756
JO - Cell
JF - Cell
IS - 5
ER -