Self-assembled Nanocomplex Using Cellulose Nanocrystal Based on Zinc/DNA Nanocluster for Gene Delivery

Hongbin Kim; Seungmin Han; Kyoungcheol Choi; Jihyeon Lee; Seung Hwan Lee; Young Wook Won; Kwang Suk Lim

doi:10.1007/s12257-022-0196-4

Self-assembled Nanocomplex Using Cellulose Nanocrystal Based on Zinc/DNA Nanocluster for Gene Delivery

Hongbin Kim, Seungmin Han, Kyoungcheol Choi, Jihyeon Lee, Seung Hwan Lee, Young Wook Won, Kwang Suk Lim

Surgery

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Cellulose nanocrystal (CNC), known as one of the most abundant terrestrial resources of nanocellulose, has excellent biocompatibility and biodegradability. In addition, since CNC has various properties such as ultrafine structure, high transparency, high purity, high crystallinity and high strength, it is being applied in various fields such as pharmaceuticals and food. In this study, we developed CNC/Zinc/DNA (CZD) nanocomplex using a method to incorporate CNC into DNA without the need for chemical conjugations by utilizing a ligand-to-metal charge transfer transition (LMCT), which occurs between divalent metal ions and the phosphate groups in CNC and DNA. Divalent metal ions should be first introduced to DNA for application of the LMCT transition to the incorporation of DNA into CNC. We optimized the conditions for CZD formation and confirmed gene transfection efficacy in the myocardial and skin cells. DNA was effectively delivered into cells by CZD and highly expressed in the cell. This complex using CNC might be useful as a gene delivery system without chemical conjugation.

Original language	English (US)
Pages (from-to)	83-90
Number of pages	8
Journal	Biotechnology and Bioprocess Engineering
Volume	28
Issue number	1
DOIs	https://doi.org/10.1007/s12257-022-0196-4
State	Published - Feb 2023

Keywords

cellulose nanocrystal
gene delivery
nanocellulose
nanocluster
zinc

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology
Biomedical Engineering

Access to Document

10.1007/s12257-022-0196-4

Cite this

@article{0b984b804eb54890b754f0da7613f12c,

title = "Self-assembled Nanocomplex Using Cellulose Nanocrystal Based on Zinc/DNA Nanocluster for Gene Delivery",

abstract = "Cellulose nanocrystal (CNC), known as one of the most abundant terrestrial resources of nanocellulose, has excellent biocompatibility and biodegradability. In addition, since CNC has various properties such as ultrafine structure, high transparency, high purity, high crystallinity and high strength, it is being applied in various fields such as pharmaceuticals and food. In this study, we developed CNC/Zinc/DNA (CZD) nanocomplex using a method to incorporate CNC into DNA without the need for chemical conjugations by utilizing a ligand-to-metal charge transfer transition (LMCT), which occurs between divalent metal ions and the phosphate groups in CNC and DNA. Divalent metal ions should be first introduced to DNA for application of the LMCT transition to the incorporation of DNA into CNC. We optimized the conditions for CZD formation and confirmed gene transfection efficacy in the myocardial and skin cells. DNA was effectively delivered into cells by CZD and highly expressed in the cell. This complex using CNC might be useful as a gene delivery system without chemical conjugation.",

keywords = "cellulose nanocrystal, gene delivery, nanocellulose, nanocluster, zinc",

author = "Hongbin Kim and Seungmin Han and Kyoungcheol Choi and Jihyeon Lee and Lee, {Seung Hwan} and Won, {Young Wook} and Lim, {Kwang Suk}",

note = "Publisher Copyright: {\textcopyright} 2023, The Korean Society for Biotechnology and Bioengineering and Springer.",

year = "2023",

month = feb,

doi = "10.1007/s12257-022-0196-4",

language = "English (US)",

volume = "28",

pages = "83--90",

journal = "Biotechnology and Bioprocess Engineering",

issn = "1226-8372",

publisher = "Korean Society for Biotechnology and Bioengineering",

number = "1",

}

TY - JOUR

T1 - Self-assembled Nanocomplex Using Cellulose Nanocrystal Based on Zinc/DNA Nanocluster for Gene Delivery

AU - Kim, Hongbin

AU - Han, Seungmin

AU - Choi, Kyoungcheol

AU - Lee, Jihyeon

AU - Lee, Seung Hwan

AU - Won, Young Wook

AU - Lim, Kwang Suk

PY - 2023/2

Y1 - 2023/2

N2 - Cellulose nanocrystal (CNC), known as one of the most abundant terrestrial resources of nanocellulose, has excellent biocompatibility and biodegradability. In addition, since CNC has various properties such as ultrafine structure, high transparency, high purity, high crystallinity and high strength, it is being applied in various fields such as pharmaceuticals and food. In this study, we developed CNC/Zinc/DNA (CZD) nanocomplex using a method to incorporate CNC into DNA without the need for chemical conjugations by utilizing a ligand-to-metal charge transfer transition (LMCT), which occurs between divalent metal ions and the phosphate groups in CNC and DNA. Divalent metal ions should be first introduced to DNA for application of the LMCT transition to the incorporation of DNA into CNC. We optimized the conditions for CZD formation and confirmed gene transfection efficacy in the myocardial and skin cells. DNA was effectively delivered into cells by CZD and highly expressed in the cell. This complex using CNC might be useful as a gene delivery system without chemical conjugation.

AB - Cellulose nanocrystal (CNC), known as one of the most abundant terrestrial resources of nanocellulose, has excellent biocompatibility and biodegradability. In addition, since CNC has various properties such as ultrafine structure, high transparency, high purity, high crystallinity and high strength, it is being applied in various fields such as pharmaceuticals and food. In this study, we developed CNC/Zinc/DNA (CZD) nanocomplex using a method to incorporate CNC into DNA without the need for chemical conjugations by utilizing a ligand-to-metal charge transfer transition (LMCT), which occurs between divalent metal ions and the phosphate groups in CNC and DNA. Divalent metal ions should be first introduced to DNA for application of the LMCT transition to the incorporation of DNA into CNC. We optimized the conditions for CZD formation and confirmed gene transfection efficacy in the myocardial and skin cells. DNA was effectively delivered into cells by CZD and highly expressed in the cell. This complex using CNC might be useful as a gene delivery system without chemical conjugation.

KW - cellulose nanocrystal

KW - gene delivery

KW - nanocellulose

KW - nanocluster

KW - zinc

UR - http://www.scopus.com/inward/record.url?scp=85146555696&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85146555696&partnerID=8YFLogxK

U2 - 10.1007/s12257-022-0196-4

DO - 10.1007/s12257-022-0196-4

M3 - Article

AN - SCOPUS:85146555696

SN - 1226-8372

VL - 28

SP - 83

EP - 90

JO - Biotechnology and Bioprocess Engineering

JF - Biotechnology and Bioprocess Engineering

IS - 1

ER -

Self-assembled Nanocomplex Using Cellulose Nanocrystal Based on Zinc/DNA Nanocluster for Gene Delivery

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this