Abstract
The flavivirus methyltransferase (MTase) sequentially methylates the N-7 and 2'-O positions of the viral RNA cap (GpppA-RNA→m7GpppA-RNA→m7GpppAm-RNA), using S-adenosyl-l-methionine (SAM) as a methyl donor. We report here the synthesis and biological evaluation of a series of novel nucleoside analogs. Two of these compounds can effectively and competitively inhibit the WNV MTase with IC50 values in micromolar range and, more importantly, do not inhibit human MTase. The compounds can also suppress the WNV replication in cell culture.
Original language | English (US) |
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Pages (from-to) | 232-239 |
Number of pages | 8 |
Journal | Antiviral Research |
Volume | 97 |
Issue number | 3 |
DOIs | |
State | Published - Mar 2013 |
Externally published | Yes |
Keywords
- Antiviral development
- Flavivirus NS5
- Methyltransferase
- RNA cap methylation
- West Nile virus
ASJC Scopus subject areas
- Pharmacology
- Virology