Black Mondo, Ophiopogon planiscapus 'Nigrescens', is an economically valuable ornamental graß creating a stunning contrast image with its almost black colour leaves. Conventional propagation by seed or division fails to meet current demand. In contrast, micropropagation can present an opportunity for the maß production in large quantities in a short time. The major goal of this study was to select the best Black Mondo clone, having the ability to simultaneously give superior shooting and rooting, among 114 genotypes for micropropagation. A kind of in vitro selection method consisting of the repeated 4 multiplication and 3 rooting cycles was performed on the in vitro germinated seedlings. Multiplication cycles were carried out in a liquid system while the rooting procedures were executed in both solidified and liquid media to compare their efficiency. Meanwhile, the effect of higher concentrated sucrose on rooting ratio was examined. Our first selection criteria was to determine the genotypes, having a multiplication rate over 3 and rooting ratio more than 95%. Then the best three clones were selected among them. Accordingly, the multiplication rates of the best three Black Mondo clones were determined to be 4.12, 3.65 and 3.27, respectively, while all of their rooting ratio was 100%. The findings revealed that Ophiopogon planiscapus 'Nigrescens' can be succeßfully micropropagated and additionally, this kind of in vitro selection method could be used efficiently for selection of the best clones either in Black Mondo, or poßibly in other plant species.