Selection of somatic hybrid plants in nicotiana through fluorescence-activated sorting of protoplasts

Claudio L. Afonso; Kristi R. Harkins; Mary A. Thomas-Compton; Ann E. Krejci; David W. Galbraith

doi:10.1038/nbt0985-811

Selection of somatic hybrid plants in nicotiana through fluorescence-activated sorting of protoplasts

Claudio L. Afonso, Kristi R. Harkins, Mary A. Thomas-Compton, Ann E. Krejci, David W. Galbraith

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43 Scopus citations

Abstract

We have developed general techniques for the successful selection of plant heterokaryons from parental populations of plant protoplasts subjected to somatic fusion treatments. These techniques involve a combination of artificial fluorescence labeling, natural chlorophyll autofluorescence and fluorescence-activated cell sorting. We have recovered purified heterokaryons at high rates and viabilities, and these were regenerated into plants. This quick and routine technique has allowed the recovery and unambiguous characterization of three different interspecific somatic hybrids of Nicotiana.

Original language	English (US)
Pages (from-to)	811-816
Number of pages	6
Journal	Bio/Technology
Volume	3
Issue number	9
DOIs	https://doi.org/10.1038/nbt0985-811
State	Published - Sep 1985
Externally published	Yes

ASJC Scopus subject areas

Biotechnology

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abstract = "We have developed general techniques for the successful selection of plant heterokaryons from parental populations of plant protoplasts subjected to somatic fusion treatments. These techniques involve a combination of artificial fluorescence labeling, natural chlorophyll autofluorescence and fluorescence-activated cell sorting. We have recovered purified heterokaryons at high rates and viabilities, and these were regenerated into plants. This quick and routine technique has allowed the recovery and unambiguous characterization of three different interspecific somatic hybrids of Nicotiana.",

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year = "1985",

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AU - Afonso, Claudio L.

AU - Harkins, Kristi R.

AU - Thomas-Compton, Mary A.

AU - Krejci, Ann E.

AU - Galbraith, David W.

PY - 1985/9

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AB - We have developed general techniques for the successful selection of plant heterokaryons from parental populations of plant protoplasts subjected to somatic fusion treatments. These techniques involve a combination of artificial fluorescence labeling, natural chlorophyll autofluorescence and fluorescence-activated cell sorting. We have recovered purified heterokaryons at high rates and viabilities, and these were regenerated into plants. This quick and routine technique has allowed the recovery and unambiguous characterization of three different interspecific somatic hybrids of Nicotiana.

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Selection of somatic hybrid plants in nicotiana through fluorescence-activated sorting of protoplasts

Abstract

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