Selection and partial characterization of a Pseudomonas aeruginosa mono- rhamnolipid deficient mutant

Marina Wild; Alma Delia Caro; Ana Lilia Hernández; Raina M. Miller; Gloria Soberón-Chávez

doi:10.1016/S0378-1097(97)00251-6

Selection and partial characterization of a Pseudomonas aeruginosa mono- rhamnolipid deficient mutant

Marina Wild, Alma Delia Caro, Ana Lilia Hernández, Raina M. Miller, Gloria Soberón-Chávez

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

Pseudomonas aeruginosa produces rhamnolipids which are tenso-active compounds with potential industrial and environmental applications. There are two main types of rhamnolipids produced in liquid cultures, rhamnosyl-β- hydroxydecanoyl-β-hydroxydecanoate (mono-rhamnolipid) and rhamnosyl- rhamnosyl-β-hydroxydecanoyl-β-hydroxydecanoate (di-rhamnolipid). In this work we report the selective isolation of a rhamnolipid deficient mutant (IBT8), which does not accumulate mono-rhamnolipid while still producing di- rhamnolipid. IBT8 was selected after random mutagenesis with Tn501; yet, its mono-rhamnolipid deficiency was found associated neither with its Tn501 insertion nor with a possible alteration in the rhlABRI genes for rhamnosyl- transferase 1 synthesis. Different possibilities to explain IBT8 phenotype are discussed.

Original language	English (US)
Pages (from-to)	279-285
Number of pages	7
Journal	FEMS Microbiology Letters
Volume	153
Issue number	2
DOIs	https://doi.org/10.1016/S0378-1097(97)00251-6
State	Published - Aug 15 1997

Keywords

Pseudomonas aeruginosa
Rhamnolipid
rhl gene

ASJC Scopus subject areas

Microbiology
Molecular Biology
Genetics

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10.1016/S0378-1097(97)00251-6

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title = "Selection and partial characterization of a Pseudomonas aeruginosa mono- rhamnolipid deficient mutant",

abstract = "Pseudomonas aeruginosa produces rhamnolipids which are tenso-active compounds with potential industrial and environmental applications. There are two main types of rhamnolipids produced in liquid cultures, rhamnosyl-β- hydroxydecanoyl-β-hydroxydecanoate (mono-rhamnolipid) and rhamnosyl- rhamnosyl-β-hydroxydecanoyl-β-hydroxydecanoate (di-rhamnolipid). In this work we report the selective isolation of a rhamnolipid deficient mutant (IBT8), which does not accumulate mono-rhamnolipid while still producing di- rhamnolipid. IBT8 was selected after random mutagenesis with Tn501; yet, its mono-rhamnolipid deficiency was found associated neither with its Tn501 insertion nor with a possible alteration in the rhlABRI genes for rhamnosyl- transferase 1 synthesis. Different possibilities to explain IBT8 phenotype are discussed.",

keywords = "Pseudomonas aeruginosa, Rhamnolipid, rhl gene",

author = "Marina Wild and Caro, {Alma Delia} and Hern{\'a}ndez, {Ana Lilia} and Miller, {Raina M.} and Gloria Sober{\'o}n-Ch{\'a}vez",

note = "Funding Information: We greatly acknowledge the support of Urs A. Ochsner during the development of this research. We thank Rebeca N{\'a}jera for her technical assistance and Fernando Bastarrachea for his critical reading of the manuscript. This research was founded in part by the United States-Mexico Foundation for Science, Grant 57-CH197, and by the National Institute of Environmental Health Sciences, Grant P42 ES04940.",

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AU - Caro, Alma Delia

AU - Hernández, Ana Lilia

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AU - Soberón-Chávez, Gloria

N1 - Funding Information: We greatly acknowledge the support of Urs A. Ochsner during the development of this research. We thank Rebeca Nájera for her technical assistance and Fernando Bastarrachea for his critical reading of the manuscript. This research was founded in part by the United States-Mexico Foundation for Science, Grant 57-CH197, and by the National Institute of Environmental Health Sciences, Grant P42 ES04940.

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Y1 - 1997/8/15

N2 - Pseudomonas aeruginosa produces rhamnolipids which are tenso-active compounds with potential industrial and environmental applications. There are two main types of rhamnolipids produced in liquid cultures, rhamnosyl-β- hydroxydecanoyl-β-hydroxydecanoate (mono-rhamnolipid) and rhamnosyl- rhamnosyl-β-hydroxydecanoyl-β-hydroxydecanoate (di-rhamnolipid). In this work we report the selective isolation of a rhamnolipid deficient mutant (IBT8), which does not accumulate mono-rhamnolipid while still producing di- rhamnolipid. IBT8 was selected after random mutagenesis with Tn501; yet, its mono-rhamnolipid deficiency was found associated neither with its Tn501 insertion nor with a possible alteration in the rhlABRI genes for rhamnosyl- transferase 1 synthesis. Different possibilities to explain IBT8 phenotype are discussed.

AB - Pseudomonas aeruginosa produces rhamnolipids which are tenso-active compounds with potential industrial and environmental applications. There are two main types of rhamnolipids produced in liquid cultures, rhamnosyl-β- hydroxydecanoyl-β-hydroxydecanoate (mono-rhamnolipid) and rhamnosyl- rhamnosyl-β-hydroxydecanoyl-β-hydroxydecanoate (di-rhamnolipid). In this work we report the selective isolation of a rhamnolipid deficient mutant (IBT8), which does not accumulate mono-rhamnolipid while still producing di- rhamnolipid. IBT8 was selected after random mutagenesis with Tn501; yet, its mono-rhamnolipid deficiency was found associated neither with its Tn501 insertion nor with a possible alteration in the rhlABRI genes for rhamnosyl- transferase 1 synthesis. Different possibilities to explain IBT8 phenotype are discussed.

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Selection and partial characterization of a Pseudomonas aeruginosa mono- rhamnolipid deficient mutant

Abstract

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